RegulonDB

RegulonDB RegulonDB 10.8: Gene Form

cysZ gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	cysZ
Synonym(s):	ECK2408, EG10003, b2413
Genome position(nucleotides):	2531463 --> 2532224 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.13
External database links:

ASAP:	ABE-0007953
ECHOBASE:	EB0003
OU-MICROARRAY:	b2413
PortEco:	cysZ
STRING:	511145.b2413
COLOMBOS:	cysZ

Product

Name:

sulfate:H⁺ symporter

Synonym(s):

CysZ

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

29.305

Isoelectric point:

9.809

Motif(s):

Type	Positions	Sequence
	75 -> 95	LLWPLAVISVLLVFGYFFSTI
	222 -> 242	IPLLNLFIMPVAVCGATAMWV
	31 -> 51	FVILPLLVNILLMGGAFWWLF
	15 -> 231	YFAQGWKLVSQPGIRRFVILPLLVNILLMGGAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAPFNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIPGIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSLFTMIPLLNLFIMP
	151 -> 171	IVLLILYFIPGIGQTVAPVLW

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.2 - sulfur metabolism
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0015116 - sulfate transmembrane transporter activity GO:0009675 - high-affinity sulfate:proton symporter activity
	biological_process	GO:0008652 - cellular amino acid biosynthetic process GO:0019344 - cysteine biosynthetic process GO:0000103 - sulfate assimilation GO:0008272 - sulfate transport GO:1902600 - proton transmembrane transport GO:1902358 - sulfate transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Assur Sanghai Z., et al., 2018
[2] Parra F., et al., 1983

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

cysZ

Operon arrangement:

Transcription unit	Promoter

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2689	2530551	reverse	nd	[RS-EPT-CBR]	[3]
promoter	cysZp1	2531383	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -3.06. P-value: 7.485e-5 Read more >	[ICWHO]	[4]
promoter	zipAp9	2531407	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.644. P-value: 5.657e-5 Read more >	[ICWHO]	[4]
promoter	TSS_2690	2531424	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_2691	2532374	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_2693 (cluster)	2532385	forward	For this promoter, there is a cluster of 3 transcription start sites,in the interval 2532385 to 2532387, where the highest frequency is at position 2530408. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]
promoter	TSS_2694 (cluster)	2532396	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2532396 to 2532397, where the highest frequency is at position 2530418. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]
promoter	TSS_2695	2533311	forward	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Assur Sanghai Z., Liu Q., Clarke OB., Belcher-Dufrisne M., Wiriyasermkul P., Giese MH., Leal-Pinto E., Kloss B., Tabuso S., Love J., Punta M., Banerjee S., Rajashankar KR., Rost B., Logothetis D., Quick M., Hendrickson WA., Mancia F., 2018, Structure-based analysis of CysZ-mediated cellular uptake of sulfate., Elife 7
[2] Parra F., Britton P., Castle C., Jones-Mortimer MC., Kornberg HL., 1983, Two separate genes involved in sulphate transport in Escherichia coli K12., J Gen Microbiol 129(2):357-8
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

RegulonDB