RegulonDB RegulonDB 11.2: Gene Form
   

aroH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

aroH ppsR ydiE TrpR TrpR terminator anti-terminator anti-anti-terminator ydiEp5 ydiEp5 TSS_1995 TSS_1995 TSS_1994 (cluster) TSS_1994 (cluster) aroHp2 aroHp2 TSS_1992 TSS_1992 aroHp1 aroHp1

Gene      
Name: aroH    Texpresso search in the literature
Synonym(s): ECK1702, EG10080, b1704
Genome position(nucleotides): 1788435 --> 1789481
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.1
External database links:  
ASAP:
 ABE-0005686
CGSC:
 1001
ECHOBASE:
 EB0078
ECOLIHUB:
 aroH
OU-MICROARRAY:
 b1704
STRING:
 511145.b1704
COLOMBOS: aroH


Product      
Name: 3-deoxy-7-phosphoheptulonate synthase, Trp-sensitive
Synonym(s): 2-dehydro-3-deoxyphosphoheptonate aldolase, AroH
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 38.735
Isoelectric point: 6.898
Motif(s):
 
Type Positions Sequence Comment
43 -> 336 EKILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGAIGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTERL
203 -> 205 RAS UniProt: In Ref. 2; AAA23493..
333 -> 333 T UniProt: In Ref. 1; AAA23497..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.15 - tryptophan
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0003849 - 3-deoxy-7-phosphoheptulonate synthase activity
GO:0042802 - identical protein binding
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009058 - biosynthetic process
GO:0009073 - aromatic amino acid family biosynthetic process
GO:0009423 - chorismate biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Ray JM., et al., 1988
External database links:  
ALPHAFOLD:
 P00887
DIP:
 DIP-6846N
ECOCYC:
 AROH-MONOMER
ECOLIWIKI:
 b1704
INTERPRO:
 IPR013785
INTERPRO:
 IPR006219
INTERPRO:
 IPR006218
MODBASE:
 P00887
PANTHER:
 PTHR21225
PFAM:
 PF00793
PRIDE:
 P00887
PRODB:
 PRO_000022149
REFSEQ:
 NP_416219
SMR:
 P00887
SWISSMODEL:
 P00887
UNIPROT:
 P00887


Operon      
Name: aroH         
Operon arrangement:
Transcription unit        Promoter
aroH
aroH


Transcriptional Regulation      
Display Regulation             
Repressed by: TrpR


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1992 1788321 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1994 (cluster) 1788419 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1995 1789271 forward nd [RS-EPT-CBR] [2]
  promoter ydiEp5 1789603 forward nd [COMP-AINF] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Ray JM., Yanofsky C., Bauerle R., 1988, Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli., J Bacteriol 170(12):5500-6

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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