RegulonDB

Gene
Name:	cheB
Synonym(s):	ECK1884, EG10147, b1883
Genome position(nucleotides):	1967452 <-- 1968501
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	54.29
External database links:

ASAP:	ABE-0006284
CGSC:	927
ECHOBASE:	EB0145
ECOLIHUB:	cheB
OU-MICROARRAY:	b1883
STRING:	511145.b1883
COLOMBOS:	cheB

Gene

Name:

cheB

Synonym(s):

ECK1884, EG10147, b1883

Genome position(nucleotides):

1967452 <-- 1968501

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

54.29

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Type

Positions

Sequence

Comment

5 -> 122

RVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYNEM

UniProt: Response regulatory.

6 -> 109

VLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAIDFVTKPQ

10 -> 10

D → N: loss of chemotactic swarmng; loss of phosphorylation and altered control of methylesterase activity in response to chemotactic stimuli (see also |CITS: [8420965]|)

11 -> 11

UniProt: Eliminates activation in response to negative stimuli, but does not affect response to positive stimuli..

42 -> 42

R → H: unphosphorylated protein has diminished methylesterase activity in vitro compared to wild type

Type

Positions

Sequence

Comment

5 -> 122

RVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYNEM

UniProt: Response regulatory.

6 -> 109

VLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAIDFVTKPQ

10 -> 10

D → N: loss of chemotactic swarmng; loss of phosphorylation and altered control of methylesterase activity in response to chemotactic stimuli (see also |CITS: [8420965]|)

11 -> 11

UniProt: Eliminates activation in response to negative stimuli, but does not affect response to positive stimuli..

42 -> 42

R → H: unphosphorylated protein has diminished methylesterase activity in vitro compared to wild type

56 -> 56

UniProt: Eliminates activation in response to negative stimuli, but does not affect response to positive stimuli..

58 -> 58

E → K: unphosphorylated protein has increased methylesterase activity in vitro compared to wild type

73 -> 73

R → H: unphosphorylated protein has diminished methylesterase activity in vitro compared to wild type

91 -> 91

E → K: unphosphorylated protein has increased methylesterase activity in vitro compared to wild type

96 -> 96

UniProt: In Ref. 1; AAA23569..

99 -> 99

L → P: loss of chemotactic swarmng; loss of phosphorylation and altered control of methylesterase activity in response to chemotactic stimuli

107 -> 107

K → R: unphosphorylated protein has diminished methylesterase activity in vitro compared to wild type

125 -> 125

UniProt: In Ref. 1; AAA23569..

152 -> 344

LLSSEKLIAIGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAVGVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQMLAKISAG

UniProt: CheB-type methylesterase.

159 -> 336

IAIGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAVGVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQ

191 -> 191

D → N: loss of chemotactic swarming; loss of methylesterase activity; can acquire phosphate group from CheA-phosphate

Multifun Terms (GenProtEC)

2 - information transfer --> 2.3 - protein related --> 2.3.3 - posttranslational modification

5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)

Gene Ontology Terms (GO)

cellular_component

GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane

molecular_function

GO:1990827 - deaminase binding
GO:0050568 - protein-glutamine glutaminase activity
GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0008984 - protein-glutamate methylesterase activity
GO:0000156 - phosphorelay response regulator activity

biological_process

GO:0006482 - protein demethylation
GO:0018277 - protein deamination
GO:0000160 - phosphorelay signal transduction system
GO:0007165 - signal transduction
GO:0006935 - chemotaxis

Transcriptional Regulation
	Display Regulation
Activated by:	FNR

Transcriptional Regulation

Display Regulation

Activated by:

FNR

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2225	1967578	reverse	nd	[RS-EPT-CBR]	[12]
promoter	TSS_2226	1967584	reverse	nd	[RS-EPT-CBR]	[12]
promoter	TSS_2227	1968082	reverse	nd	[RS-EPT-CBR]	[12]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

TSS_2225

1967578

reverse

[RS-EPT-CBR]

[12]

promoter

TSS_2226

1967584

reverse

[RS-EPT-CBR]

[12]

promoter

TSS_2227

1968082

reverse

[RS-EPT-CBR]

[12]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Evidence

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Banno S., Shiomi D., Homma M., Kawagishi I., 2004, Targeting of the chemotaxis methylesterase/deamidase CheB to the polar receptor-kinase cluster in an Escherichia coli cell., Mol Microbiol 53(4):1051-63
[2] Dang CV., Niwano M., Ryu J., Taylor BL., 1986, Inversion of aerotactic response in Escherichia coli deficient in cheB protein methylesterase., J Bacteriol 166(1):275-80
[3] Kehry MR., Doak TG., Dahlquist FW., 1985, Sensory adaptation in bacterial chemotaxis: regulation of demethylation., J Bacteriol 163(3):983-90
[4] Lai WC., Hazelbauer GL., 2005, Carboxyl-terminal extensions beyond the conserved pentapeptide reduce rates of chemoreceptor adaptational modification., J Bacteriol 187(15):5115-21
[5] Parkinson JS., 1978, Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis., J Bacteriol 135(1):45-53
[6] Rollins CM., Dahlquist FW., 1980, Methylation of chemotaxis-specific proteins in Escherichia coli cells permeable to S-adenosylmethionine., Biochemistry 19(20):4627-32
[7] Russell CB., Stewart RC., Dahlquist FW., 1989, Control of transducer methylation levels in Escherichia coli: investigation of components essential for modulation of methylation and demethylation reactions., J Bacteriol 171(7):3609-18
[8] Saxl RL., Anand GS., Stock AM., 2001, Synthesis and biochemical characterization of a phosphorylated analogue of the response regulator CheB., Biochemistry 40(43):12896-903
[9] Sherris D., Parkinson JS., 1981, Posttranslational processing of methyl-accepting chemotaxis proteins in Escherichia coli., Proc Natl Acad Sci U S A 78(10):6051-5
[10] Stewart RC., Dahlquist FW., 1988, N-terminal half of CheB is involved in methylesterase response to negative chemotactic stimuli in Escherichia coli., J Bacteriol 170(12):5728-38
[11] Stewart RC., Russell CB., Roth AF., Dahlquist FW., 1988, Interaction of CheB with chemotaxis signal transduction components in Escherichia coli: modulation of the methylesterase activity and effects on cell swimming behavior., Cold Spring Harb Symp Quant Biol 53 Pt 1:27-40
[12] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Banno S., Shiomi D., Homma M., Kawagishi I., 2004, Targeting of the chemotaxis methylesterase/deamidase CheB to the polar receptor-kinase cluster in an Escherichia coli cell., Mol Microbiol 53(4):1051-63

[2] Dang CV., Niwano M., Ryu J., Taylor BL., 1986, Inversion of aerotactic response in Escherichia coli deficient in cheB protein methylesterase., J Bacteriol 166(1):275-80

[3] Kehry MR., Doak TG., Dahlquist FW., 1985, Sensory adaptation in bacterial chemotaxis: regulation of demethylation., J Bacteriol 163(3):983-90

[4] Lai WC., Hazelbauer GL., 2005, Carboxyl-terminal extensions beyond the conserved pentapeptide reduce rates of chemoreceptor adaptational modification., J Bacteriol 187(15):5115-21

[5] Parkinson JS., 1978, Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis., J Bacteriol 135(1):45-53

[6] Rollins CM., Dahlquist FW., 1980, Methylation of chemotaxis-specific proteins in Escherichia coli cells permeable to S-adenosylmethionine., Biochemistry 19(20):4627-32

[7] Russell CB., Stewart RC., Dahlquist FW., 1989, Control of transducer methylation levels in Escherichia coli: investigation of components essential for modulation of methylation and demethylation reactions., J Bacteriol 171(7):3609-18

[8] Saxl RL., Anand GS., Stock AM., 2001, Synthesis and biochemical characterization of a phosphorylated analogue of the response regulator CheB., Biochemistry 40(43):12896-903

[9] Sherris D., Parkinson JS., 1981, Posttranslational processing of methyl-accepting chemotaxis proteins in Escherichia coli., Proc Natl Acad Sci U S A 78(10):6051-5

[10] Stewart RC., Dahlquist FW., 1988, N-terminal half of CheB is involved in methylesterase response to negative chemotactic stimuli in Escherichia coli., J Bacteriol 170(12):5728-38

[11] Stewart RC., Russell CB., Roth AF., Dahlquist FW., 1988, Interaction of CheB with chemotaxis signal transduction components in Escherichia coli: modulation of the methylesterase activity and effects on cell swimming behavior., Cold Spring Harb Symp Quant Biol 53 Pt 1:27-40

[12] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.