RegulonDB RegulonDB 10.10: Gene Form
   

cheW gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cheA tar cheW C0465 TSS_2228 TSS_2228 cheWp5 cheWp5 tarp tarp

Gene      
Name: cheW    Texpresso search in the literature
Synonym(s): ECK1888, EG10149, b1887
Genome position(nucleotides): 1972836 <-- 1973339 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.98
External database links:  
ASAP:
 ABE-0006292
CGSC:
 925
ECHOBASE:
 EB0147
ECOLIHUB:
 cheW
OU-MICROARRAY:
 b1887
STRING:
 511145.b1887
COLOMBOS: cheW


Product      
Name: chemotaxis protein CheW
Synonym(s): CheW, purine-binding chemotaxis protein
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,cytosol
Molecular weight: 18.084
Isoelectric point: 4.062
Motif(s):
 
Type Positions Sequence
56 -> 56 K
57 -> 57 G
36 -> 36 V
133 -> 133 G
158 -> 158 L

 
Classification:
Multifun Terms (GenProtEC)  
  3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.3 - inhibition / activation of enzymes
  5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
Gene Ontology Terms (GO)  
cellular_component GO:0098561 - methyl accepting chemotaxis protein complex
GO:0051286 - cell tip
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane
molecular_function GO:0019904 - protein domain specific binding
GO:0005515 - protein binding
GO:0042803 - protein homodimerization activity
biological_process GO:1901875 - positive regulation of post-translational protein modification
GO:0010864 - positive regulation of protein histidine kinase activity
GO:0007165 - signal transduction
GO:0006935 - chemotaxis
GO:0051649 - establishment of localization in cell
GO:0009454 - aerotaxis
Note(s): Note(s): ...[more].
Evidence: [APPH] Assay of protein purified to homogeneity
[AUP] Assay of unpurified protein
[IMP] Inferred from mutant phenotype
Reference(s): [1] Cashman DJ., et al., 2013
[2] Cassidy CK., et al., 2020
[3] Gegner JA., et al., 1992
[4] Liu JD., et al., 1991
[5] Liu JD., et al., 1989
[6] Maddock JR., et al., 1993
[7] Mutoh N., et al., 1986
[8] Parkinson JS. 1978
[9] Sourjik V., et al., 2000
[10] Underbakke ES., et al., 2011
[11] Wolfe AJ., et al., 1987
External database links:  
DIP:
 DIP-48236N
ECOCYC:
 CHEW-MONOMER
ECOLIWIKI:
 b1887
INTERPRO:
 IPR002545
INTERPRO:
 IPR039315
INTERPRO:
 IPR036061
MODBASE:
 P0A964
PANTHER:
 PTHR22617
PDB:
 2HO9
PFAM:
 PF01584
PRIDE:
 P0A964
PRODB:
 PRO_000022280
PROSITE:
 PS50851
REFSEQ:
 NP_416401
SMART:
 SM00260
SMR:
 P0A964
UNIPROT:
 P0A964


Operon      
Name: motAB-cheAW         
Operon arrangement:
Transcription unit        Promoter
motAB-cheAW


Transcriptional Regulation      
Display Regulation             
Repressed by: CpxR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter cheWp5 1973462 reverse nd [ICWHO] [12]
  promoter TSS_2228 1973726 reverse nd [RS-EPT-CBR] [13]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Cashman DJ., Ortega DR., Zhulin IB., Baudry J., 2013, Homology modeling of the CheW coupling protein of the chemotaxis signaling complex., PLoS One 8(8):e70705

 [2] Cassidy CK., Himes BA., Sun D., Ma J., Zhao G., Parkinson JS., Stansfeld PJ., Luthey-Schulten Z., Zhang P., 2020, Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations., Commun Biol 3(1):24

 [3] Gegner JA., Graham DR., Roth AF., Dahlquist FW., 1992, Assembly of an MCP receptor, CheW, and kinase CheA complex in the bacterial chemotaxis signal transduction pathway., Cell 70(6):975-82

 [4] Liu JD., Parkinson JS., 1991, Genetic evidence for interaction between the CheW and Tsr proteins during chemoreceptor signaling by Escherichia coli., J Bacteriol 173(16):4941-51

 [5] Liu JD., Parkinson JS., 1989, Role of CheW protein in coupling membrane receptors to the intracellular signaling system of bacterial chemotaxis., Proc Natl Acad Sci U S A 86(22):8703-7

 [6] Maddock JR., Shapiro L., 1993, Polar location of the chemoreceptor complex in the Escherichia coli cell., Science 259(5102):1717-23

 [7] Mutoh N., Simon MI., 1986, Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli., J Bacteriol 165(1):161-6

 [8] Parkinson JS., 1978, Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis., J Bacteriol 135(1):45-53

 [9] Sourjik V., Berg HC., 2000, Localization of components of the chemotaxis machinery of Escherichia coli using fluorescent protein fusions., Mol Microbiol 37(4):740-51

 [10] Underbakke ES., Zhu Y., Kiessling LL., 2011, Protein footprinting in a complex milieu: identifying the interaction surfaces of the chemotaxis adaptor protein CheW., J Mol Biol 409(4):483-95

 [11] Wolfe AJ., Conley MP., Kramer TJ., Berg HC., 1987, Reconstitution of signaling in bacterial chemotaxis., J Bacteriol 169(5):1878-85

 [12] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [13] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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