RegulonDB RegulonDB 10.8: Gene Form
   

era gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

lepB era recO rnc Cis-reg rncp rncp TSS_2865 (cluster) TSS_2865 (cluster) TSS_2864 TSS_2864 TSS_2863 TSS_2863 TSS_2862 TSS_2862 TSS_2861 TSS_2861 TSS_2860 (cluster) TSS_2860 (cluster) TSS_2859 TSS_2859 TSS_2858 TSS_2858 TSS_2857 TSS_2857 TSS_2856 TSS_2856 pdxJp pdxJp

Gene      
Name: era    Texpresso search in the literature
Synonym(s): ECK2564, EG10270, b2566, rbaA, sdgE
Genome position(nucleotides): 2702481 <-- 2703386 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.32
External database links:  
ASAP:
 ABE-0008440
CGSC:
 29010
ECHOBASE:
 EB0266
OU-MICROARRAY:
 b2566
PortEco:
 era
STRING:
 511145.b2566
COLOMBOS: era


Product      
Name: 30S ribosomal subunit maturation GTPase Era
Synonym(s): Era, RbaA, SdgE
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,cytosol
Molecular weight: 33.81
Isoelectric point: 7.293
Motif(s):
 
Type Positions Sequence
17 -> 17 P
62 -> 66 DTPGL
124 -> 127 NKVD
156 -> 156 A
2 -> 301 SIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSLGYVDDL

 
Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.2 - cell cycle physiology
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0031234 - extrinsic component of cytoplasmic side of plasma membrane
molecular_function GO:0005515 - protein binding
GO:0003723 - RNA binding
GO:0000166 - nucleotide binding
GO:0003924 - GTPase activity
GO:0019843 - rRNA binding
GO:0005525 - GTP binding
GO:0070181 - small ribosomal subunit rRNA binding
GO:0043024 - ribosomal small subunit binding
GO:0097216 - guanosine tetraphosphate binding
biological_process GO:0042254 - ribosome biogenesis
GO:0006468 - protein phosphorylation
GO:0046777 - protein autophosphorylation
GO:0042274 - ribosomal small subunit biogenesis
GO:0000028 - ribosomal small subunit assembly
Note(s): Note(s): ...[more].
Evidence: [IDA] Inferred from direct assay
[IMP] Inferred from mutant phenotype
Reference(s): [1] Anantharaman V., et al., 2002
[2] Ash MR., et al., 2012
[3] Britton RA., et al., 1997
[4] Bunner AE., et al., 2010
[5] Chen S., et al., 1991
[6] Gollop N., et al., 1991
[7] Huang Y., et al., 2007
[8] Inoue K., et al., 2003
[9] Inoue K., et al., 2002
[10] Inoue K., et al., 2006
[11] Lerner CG., et al., 1995
[12] Lerner CG., et al., 1992
[13] Lin YP., et al., 1994
[14] Pillutla RC., et al., 1996
[15] Pillutla RC., et al., 1995
[16] Powell BS., et al., 1995
[17] Rafay A., et al., 2012
[18] Sayed A., et al., 2000
[19] Sood P., et al., 1994
[20] Valencia A., et al., 1991
[21] Wang X., et al., 2012
[22] Watt RM., et al., 2007
External database links:  
DIP:
 DIP-9521N
ECOCYC:
 EG10270-MONOMER
ECOLIWIKI:
 b2566
INTERPRO:
 IPR005662
INTERPRO:
 IPR030388
INTERPRO:
 IPR027417
INTERPRO:
 IPR015946
INTERPRO:
 IPR009019
INTERPRO:
 IPR006073
INTERPRO:
 IPR005225
INTERPRO:
 IPR004044
MINT:
 MINT-1248282
MODBASE:
 P06616
PDB:
 3IEU
PDB:
 1EGA
PFAM:
 PF01926
PFAM:
 PF07650
PRIDE:
 P06616
PRODB:
 PRO_000024943
PROSITE:
 PS51713
PROSITE:
 PS50823
REFSEQ:
 NP_417061
SMR:
 P06616
UNIPROT:
 P06616


Operon      
Name: rnc-era-recO-pdxJ-acpS         
Operon arrangement:
Transcription unit        Promoter
pdxJ-acpS
rnc-era
rnc-era-recO-pdxJ-acpS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2856 2702292 reverse nd [RS-EPT-CBR] [23]
  promoter TSS_2857 2702302 reverse nd [RS-EPT-CBR] [23]
  promoter TSS_2858 2703503 reverse nd [RS-EPT-CBR] [23]
  promoter TSS_2859 2704008 reverse nd [RS-EPT-CBR] [23]
  promoter TSS_2860 (cluster) 2704016 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [23]
  promoter TSS_2861 2704074 reverse nd [RS-EPT-CBR] [23]
  promoter TSS_2862 2704076 reverse nd [RS-EPT-CBR] [23]
  promoter TSS_2863 2704093 reverse nd [RS-EPT-CBR] [23]
  promoter TSS_2864 2704101 reverse nd [RS-EPT-CBR] [23]
  promoter TSS_2865 (cluster) 2704183 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [23]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Anantharaman V., Koonin EV., Aravind L., 2002, Comparative genomics and evolution of proteins involved in RNA metabolism., Nucleic Acids Res 30(7):1427-64

 [2] Ash MR., Maher MJ., Mitchell Guss J., Jormakka M., 2012, The cation-dependent G-proteins: In a class of their own., FEBS Lett 586(16):2218-24

 [3] Britton RA., Lupski JR., 1997, Isolation and characterization of suppressors of two Escherichia coli dnaG mutations, dnaG2903 and parB., Genetics 145(4):867-75

 [4] Bunner AE., Nord S., Wikstrom PM., Williamson JR., 2010, The effect of ribosome assembly cofactors on in vitro 30S subunit reconstitution., J Mol Biol 398(1):1-7

 [5] Chen S., Court DL., 1991, High expression of era gene., Chin J Biotechnol 7(3):177-84

 [6] Gollop N., March PE., 1991, Localization of the membrane binding sites of Era in Escherichia coli., Res Microbiol 142(2-3):301-7

 [7] Huang Y., Zhang B., Dong K., Zhang X., Hou L., Wang T., Chen N., Chen S., 2007, Up-regulation of yggG promotes the survival of Escherichia coli cells containing Era-1 mutant protein., FEMS Microbiol Lett 275(1):8-15

 [8] Inoue K., Alsina J., Chen J., Inouye M., 2003, Suppression of defective ribosome assembly in a rbfA deletion mutant by overexpression of Era, an essential GTPase in Escherichia coli., Mol Microbiol 48(4):1005-16

 [9] Inoue K., Chen J., Kato I., Inouye M., 2002, Specific growth inhibition by acetate of an Escherichia coli strain expressing Era-dE, a dominant negative Era mutant., J Mol Microbiol Biotechnol 4(4):379-88

 [10] Inoue K., Chen J., Tan Q., Inouye M., 2006, Era and RbfA have overlapping function in ribosome biogenesis in Escherichia coli., J Mol Microbiol Biotechnol 11(1-2):41-52

 [11] Lerner CG., Gulati PS., Inouye M., 1995, Cold-sensitive conditional mutations in Era, an essential Escherichia coli GTPase, isolated by localized random polymerase chain reaction mutagenesis., FEMS Microbiol Lett 126(3):291-8

 [12] Lerner CG., Sood P., Ahnn J., Inouye M., 1992, Cold-sensitive growth and decreased GTP-hydrolytic activity from substitution of Pro17 for Val in Era, an essential Escherichia coli GTPase., FEMS Microbiol Lett 74(2-3):137-42

 [13] Lin YP., Sharer JD., March PE., 1994, GTPase-dependent signaling in bacteria: characterization of a membrane-binding site for era in Escherichia coli., J Bacteriol 176(1):44-9

 [14] Pillutla RC., Ahnn J., Inouye M., 1996, Deletion of the putative effector region of Era, an essential GTP-binding protein in Escherichia coli, causes a dominant-negative phenotype., FEMS Microbiol Lett 143(1):47-55

 [15] Pillutla RC., Sharer JD., Gulati PS., Wu E., Yamashita Y., Lerner CG., Inouye M., March PE., 1995, Cross-species complementation of the indispensable Escherichia coli era gene highlights amino acid regions essential for activity., J Bacteriol 177(8):2194-6

 [16] Powell BS., Court DL., Inada T., Nakamura Y., Michotey V., Cui X., Reizer A., Saier MH., Reizer J., 1995, Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant., J Biol Chem 270(9):4822-39

 [17] Rafay A., Majumdar S., Prakash B., 2012, Exploring potassium-dependent GTP hydrolysis in TEES family GTPases., FEBS Open Bio 2:173-7

 [18] Sayed A., Matsuyama S., Inoue K., Alsina J., Cai F., Chen J., Inouye M., 2000, ATPase and GTPase activities copurifying with GTP-binding proteins in E. coli., J Mol Microbiol Biotechnol 2(3):261-3

 [19] Sood P., Lerner CG., Shimamoto T., Lu Q., Inouye M., 1994, Characterization of the autophosphorylation of Era, an essential Escherichia coli GTPase., Mol Microbiol 12(2):201-8

 [20] Valencia A., Chardin P., Wittinghofer A., Sander C., 1991, The ras protein family: evolutionary tree and role of conserved amino acids., Biochemistry 30(19):4637-48

 [21] Wang X., Xue J., Sun Z., Qin Y., Gong W., 2012, Study on the chaperone properties of conserved GTPases., Protein Cell 3(1):44-50

 [22] Watt RM., Wang J., Leong M., Kung HF., Cheah KS., Liu D., Danchin A., Huang JD., 2007, Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes., Nucleic Acids Res 35(6):e37

 [23] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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