RegulonDB

Gene
Name:	fabA
Synonym(s):	ECK0945, EG10273, b0954
Genome position(nucleotides):	1015952 <-- 1016470 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.18
Reference(s):	[1] Cronan JE., et al., 1988 [2] Cronan JE., et al., 1972 [3] Feng Y., et al., 2009 [4] Feng Y., et al., 2011 [5] Henry MF., et al., 1992 [6] Nunn WD., et al., 1983
External database links:

ASAP:	ABE-0003229
CGSC:	799
ECHOBASE:	EB0269
ECOLIHUB:	fabA
OU-MICROARRAY:	b0954
STRING:	511145.b0954
COLOMBOS:	fabA

Product

Name:

β-hydroxyacyl-acyl carrier protein dehydratase/isomerase

Synonym(s):

beta;-hydroxyacyl-ACP dehydratase/isomerase, FabA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

18.969

Isoelectric point:

6.532

Motif(s):

Type	Positions	Sequence
	2 -> 172	VDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMTETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEGKGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASDLKVGLFQDTSAF
	29 -> 158	LPAPNMLMMDRVVKMTETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEGKGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTA
	102 -> 102	G
	76 -> 76	P

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.4 - fatty acids and phosphatidic acid
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0008659 - (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity GO:0004317 - 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity GO:0016829 - lyase activity GO:0016853 - isomerase activity GO:0008693 - 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity GO:0019171 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity GO:0042803 - protein homodimerization activity GO:0034017 - trans-2-decenoyl-acyl-carrier-protein isomerase activity GO:0047451 - 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity
	biological_process	GO:0006629 - lipid metabolic process GO:0006631 - fatty acid metabolic process GO:0008610 - lipid biosynthetic process GO:0006633 - fatty acid biosynthetic process

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PANTHER:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

fabA

Operon arrangement:

Transcription unit	Promoter
fabA	fabAp

Transcriptional Regulation
	Display Regulation
Activated by:	FadR
Repressed by:	FabR

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_1249	1016472	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1250	1016488	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1251 (cluster)	1016497	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1252	1016859	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1253	1017045	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1254	1017052	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1255 (cluster)	1017834	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1256	1017837	reverse	nd	[RS-EPT-CBR]	[7]
promoter	TSS_1257	1017843	reverse	nd	[RS-EPT-CBR]	[7]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Cronan JE., Li WB., Coleman R., Narasimhan M., de Mendoza D., Schwab JM., 1988, Derived amino acid sequence and identification of active site residues of Escherichia coli beta-hydroxydecanoyl thioester dehydrase., J Biol Chem 263(10):4641-6
[2] Cronan JE., Silbert DF., Wulff DL., 1972, Mapping of the fabA locus for unsaturated fatty acid biosynthesis in Escherichia coli., J Bacteriol 112(1):206-11
[3] Feng Y., Cronan JE., 2009, Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabB., J Biol Chem 284(43):29526-35
[4] Feng Y., Cronan JE., 2011, Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters., Mol Microbiol 80(1):195-218
[5] Henry MF., Cronan JE., 1992, A new mechanism of transcriptional regulation: release of an activator triggered by small molecule binding., Cell 70(4):671-9
[6] Nunn WD., Giffin K., Clark D., Cronan JE., 1983, Role for fadR in unsaturated fatty acid biosynthesis in Escherichia coli., J Bacteriol 154(2):554-60
[7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.