RegulonDB RegulonDB 11.1: Gene Form
   

fhuA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mrcB fhuA fhuC Fur terminator TSS_289 TSS_289 fhuAp fhuAp

Gene      
Name: fhuA    Texpresso search in the literature
Synonym(s): ECK0149, EG10302, T1, T5rec, b0150, tonA
Genome position(nucleotides): 167484 --> 169727
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.25
External database links:  
ASAP:
 ABE-0000520
CGSC:
 777
ECHOBASE:
 EB0298
ECOLIHUB:
 fhuA
OU-MICROARRAY:
 b0150
STRING:
 511145.b0150
COLOMBOS: fhuA


Product      
Name: ferrichrome outer membrane transporter/phage receptor
Synonym(s): FhuA, TonA
Sequence: Get amino acid sequence Fasta Format
Cellular location: outer membrane
Molecular weight: 82.182
Isoelectric point: 5.359
Motif(s):
 
Type Positions Sequence Comment
1 -> 33 MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQA
34 -> 747 AVEPKEDTITVTAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEALSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLERAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLDDDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGYYGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLRFAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDTQLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDPANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQFTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRPIVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVVATATFRF UniProt: Ferrichrome outer membrane transporter/phage receptor.
40 -> 47 DTITVTAA UniProt: TonB box; Sequence Annotation Type: short sequence motif.
77 -> 180 QKVPQSISVVTAEEMALHQPKSVKEALSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLERAEIMRGPVSVLYGKSSPGG
148 -> 149 FY UniProt: Ferrichrome binding; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.1 - Channel-type Transporters --> 4.1.B - Beta barrel porins (The Outer Membrane Porin (OMP) Functional Superfamily)
  8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
  8 - extrachromosomal --> 8.4 - colicin related
Gene Ontology Terms (GO)  
cellular_component GO:0009279 - cell outer membrane
GO:0016020 - membrane
GO:0016021 - integral component of membrane
GO:1902495 - transmembrane transporter complex
molecular_function GO:0019904 - protein domain specific binding
GO:0005515 - protein binding
GO:0015643 - toxic substance binding
GO:0005506 - iron ion binding
GO:0046790 - virion binding
GO:0038023 - signaling receptor activity
GO:0015344 - siderophore uptake transmembrane transporter activity
biological_process GO:0006811 - ion transport
GO:0055072 - iron ion homeostasis
GO:0015891 - siderophore transport
GO:0044718 - siderophore transmembrane transport
Note(s): Note(s): ...[more].
Evidence: [EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Braun M., et al., 2002
[2] Li Z., et al., 2021
[3] Samsonov VV., et al., 2002
[4] Thoma J., et al., 2018
External database links:  
ALPHAFOLD:
 P06971
DIP:
 DIP-9602N
ECOCYC:
 EG10302-MONOMER
ECOLIWIKI:
 b0150
INTERPRO:
 IPR012910
INTERPRO:
 IPR010105
INTERPRO:
 IPR000531
INTERPRO:
 IPR010916
INTERPRO:
 IPR036942
INTERPRO:
 IPR010917
INTERPRO:
 IPR039423
INTERPRO:
 IPR037066
MODBASE:
 P06971
PANTHER:
 PTHR32552
PDB:
 4CU4
PDB:
 2GRX
PDB:
 2FCP
PDB:
 1QKC
PDB:
 1QJQ
PDB:
 1QFG
PDB:
 1QFF
PDB:
 1FI1
PDB:
 1FCP
PDB:
 1BY3
PDB:
 1BY5
PFAM:
 PF07715
PFAM:
 PF00593
PRIDE:
 P06971
PRODB:
 PRO_000022609
PROSITE:
 PS01156
PROSITE:
 PS00430
REFSEQ:
 NP_414692
SMR:
 P06971
UNIPROT:
 P06971


Operon      
Name: fhuACDB         
Operon arrangement:
Transcription unit        Promoter
fhuACDB


Transcriptional Regulation      
Display Regulation             
Repressed by: Fur


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_289 169733 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Braun M., Killmann H., Maier E., Benz R., Braun V., 2002, Diffusion through channel derivatives of the Escherichia coli FhuA transport protein., Eur J Biochem 269(20):4948-59

 [2] Li Z., Chinnasamy S., Zhang Y., Wei DQ., 2021, Molecular dynamics simulation and binding free energy calculations of microcin J25 binding to the FhuA receptor., J Biomol Struct Dyn 39(7):2585-2594

 [3] Samsonov VV., Samsonov VV., Sineoky SP., 2002, DcrA and dcrB Escherichia coli genes can control DNA injection by phages specific for BtuB and FhuA receptors., Res Microbiol 153(10):639-46

 [4] Thoma J., Manioglu S., Kalbermatter D., Bosshart PD., Fotiadis D., Muller DJ., 2018, Protein-enriched outer membrane vesicles as a native platform for outer membrane protein studies., Commun Biol 1:23

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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