RegulonDB

RegulonDB RegulonDB 10.9: Gene Form

fumC gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	fumC
Synonym(s):	ECK1606, EG10358, b1611
Genome position(nucleotides):	1685185 <-- 1686588 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.77
External database links:

ASAP:	ABE-0005380
CGSC:	15273
ECHOBASE:	EB0353
ECOLIHUB:	fumC
MIM:	606812
MIM:	136850
MIM:	150800
OU-MICROARRAY:	b1611
STRING:	511145.b1611
COLOMBOS:	fumC

Product

Name:

fumarase C

Synonym(s):

FumC

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

50.489

Isoelectric point:

6.561

Motif(s):

Type	Positions	Sequence
	12 -> 342	GAIDVPADKLWGAQTQRSLEHFRISTEKMPTSLIHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQSNMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQLIPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYSLPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQAHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALTMLCCQVMG
	188 -> 188	H
	127 -> 127	K
	408 -> 461	LVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEFDSWVRPEQMVG
	98 -> 100	SGT

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0045239 - tricarboxylic acid cycle enzyme complex
	molecular_function	GO:0003824 - catalytic activity GO:0016829 - lyase activity GO:0004333 - fumarate hydratase activity GO:0042802 - identical protein binding
	biological_process	GO:0008152 - metabolic process GO:0006099 - tricarboxylic acid cycle GO:0006979 - response to oxidative stress GO:0006108 - malate metabolic process GO:0006106 - fumarate metabolic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Calderon IL., et al., 2009
[2] Chen YP., et al., 2012
[3] Guest JR., et al., 1985
[4] Woods SA., et al., 1986

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

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REFSEQ:

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UNIPROT:

Operon

Name:

fumAC

Operon arrangement:

Transcription unit	Promoter
fumC	fumCp1
fumC	fumCp
fumA	fumAp
fumAC	fumAp

Transcriptional Regulation

Display Regulation

Activated by:

SoxR, MarA, CRP, Rob, SoxS

Repressed by:

FNR, Fur, ArcA

Undetermined regulation

CRP

Growth Conditions:

[1]	C: Escherichia coli\| soxR knockout mutant; soxS knockout mutant\| LB medium\| 37.0 C E: Escherichia coli\| soxR knockout mutant; soxS knockout mutant; plasmid pSXR (not induced soxR) mutant\| LB medium\| 37.0 C
[2]	C: Escherichia coli\| soxR knockout mutant; soxS knockout mutant\| LB medium\| 37.0 C E: Escherichia coli\| soxR knockout mutant; soxS knockout mutant; plasmid pSXR (IPTG-induced soxR) mutant\| LB medium\| 37.0 C

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	tusp2	1684173		forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 0.96. P-value: 2.991e-6 Read more >	[ICWHO]	[5]

Evidence
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Calderon IL., Elias AO., Fuentes EL., Pradenas GA., Castro ME., Arenas FA., Perez JM., Vasquez CC., 2009, Tellurite-mediated disabling of [4Fe-4S] clusters of Escherichia coli dehydratases., Microbiology 155(Pt 6):1840-6
[2] Chen YP., Lin HH., Yang CD., Huang SH., Tseng CP., 2012, Regulatory role of cAMP receptor protein over Escherichia coli fumarase genes., J Microbiol 50(3):426-33
[3] Guest JR., Miles JS., Roberts RE., Woods SA., 1985, The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC)., J Gen Microbiol 131(11):2971-84
[4] Woods SA., Miles JS., Roberts RE., Guest JR., 1986, Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12., Biochem J 237(2):547-57
[5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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