RegulonDB

Gene
Name:	galS
Synonym(s):	ECK2144, EG10365, b2151, mglD
Genome position(nucleotides):	2240628 <-- 2241668
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.45
External database links:

ASAP:	ABE-0007110
CGSC:	499
ECHOBASE:	EB0360
ECOLIHUB:	galS
OU-MICROARRAY:	b2151
STRING:	511145.b2151
COLOMBOS:	galS

Shine dalgarno
Sequence:	atctcataacAGGTAGtgaaTAT

Product

Name:

DNA-binding transcriptional dual regulator GalS

Synonym(s):

GalS, MglD

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

GalR/LacI

Cellular location:

cytosol

Molecular weight:

37.357

Isoelectric point:

6.678

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 56	MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALAT	UniProt: HTH lacI-type.
	3 -> 48	TIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPN
	4 -> 23	IRDVARQAGVSVATVSRVLN	UniProt: H-T-H motif.
	104 -> 105	ER	UniProt: In Ref. 1; CAA44390..
	169 -> 329	RMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGAGTPDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSV

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding GO:0003700 - DNA-binding transcription factor activity GO:0000976 - transcription cis-regulatory region binding
	biological_process	GO:0006355 - regulation of transcription, DNA-templated

Note(s):

Note(s): ...[more].

Evidence:

[COMP-HINF-FN-FROM-SEQ] Human inference of function from sequence
[EXP-IDA-PURIFIED-PROTEIN] Assay of protein purified to homogeneity
[EXP-IEP] Inferred from expression pattern
[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Geanacopoulos M., et al., 1997
[2] Vermes I., et al., 1991
[3] Weickert MJ., et al., 1992
[4] Weickert MJ., et al., 1993
[5] Weickert MJ., et al., 1993

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	CRP
Repressed by:	GalS, GalR

Reference(s)
[1] Geanacopoulos M., Adhya S., 1997, Functional characterization of roles of GalR and GalS as regulators of the gal regulon., J Bacteriol 179(1):228-34
[2] Vermes I., Bonte HA., v d Sluijs Veer G., Schoemaker J., 1991, Interpretations of five monoclonal immunoassays of lutropin and follitropin: effects of normalization with WHO standard., Clin Chem 37(3):415-21
[3] Weickert MJ., Adhya S., 1992, Isorepressor of the gal regulon in Escherichia coli., J Mol Biol 226(1):69-83
[4] Weickert MJ., Adhya S., 1993, The galactose regulon of Escherichia coli., Mol Microbiol 10(2):245-51
[5] Weickert MJ., Adhya S., 1993, Control of transcription of gal repressor and isorepressor genes in Escherichia coli., J Bacteriol 175(1):251-8