RegulonDB RegulonDB 11.1: Gene Form
   

gshA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

gshA yqaA micA yqaB TSS_3001 TSS_3001 TSS_3000 TSS_3000 TSS_2999 TSS_2999 TSS_2998 TSS_2998 luxSp1 luxSp1 luxSp2 luxSp2 micAp micAp

Gene      
Name: gshA    Texpresso search in the literature
Synonym(s): ECK2683, EG10418, b2688, gsh-I
Genome position(nucleotides): 2814883 <-- 2816439
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.32
External database links:  
ASAP:
 ABE-0008845
CGSC:
 659
ECHOBASE:
 EB0413
ECOLIHUB:
 gshA
OU-MICROARRAY:
 b2688
STRING:
 511145.b2688
COLOMBOS: gshA


Product      
Name: glutamate—cysteine ligase
Synonym(s): gamma;-glutamatemdash;cysteine ligase, GshA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 58.269
Isoelectric point: 5.041
Motif(s):
 
Type Positions Sequence Comment
10 -> 380 AWLEKHPQALKGIQRGLERETLRVNADGTLATTGHPEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPLSMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPFEKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRV
100 -> 100 W UniProt: In Ref. 1; CAA27583..
494 -> 495 AS UniProt: In Ref. 1; CAA27583..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.10 - glutathione
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004357 - glutamate-cysteine ligase activity
biological_process GO:0006750 - glutathione biosynthetic process
GO:0006972 - hyperosmotic response
GO:0071243 - cellular response to arsenic-containing substance
GO:0071288 - cellular response to mercury ion
Note(s): Note(s): ...[more].
Reference(s): [1] Prinz WA., et al., 1997
[2] Smirnova GV., et al., 2001
External database links:  
ALPHAFOLD:
 P0A6W9
DIP:
 DIP-48212N
ECOCYC:
 GLUTCYSLIG-MONOMER
ECOLIWIKI:
 b2688
INTERPRO:
 IPR014746
INTERPRO:
 IPR007370
INTERPRO:
 IPR006334
PANTHER:
 PTHR38761
PDB:
 2D33
PDB:
 2D32
PDB:
 1V4G
PDB:
 1VA6
PFAM:
 PF04262
PRIDE:
 P0A6W9
PRODB:
 PRO_000022843
REFSEQ:
 NP_417173
SMR:
 P0A6W9
UNIPROT:
 P0A6W9


Operon      
Name: gshA         
Operon arrangement:
Transcription unit        Promoter
gshA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2998 2815236 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2999 2815242 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3000 2816260 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_3001 2816461 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Prinz WA., Aslund F., Holmgren A., Beckwith J., 1997, The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm., J Biol Chem 272(25):15661-7

 [2] Smirnova GV., Krasnykh TA., Oktyabrsky ON., 2001, Role of glutathione in the response of Escherichia coli to osmotic stress., Biochemistry (Mosc) 66(9):973-8

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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