RegulonDB RegulonDB 11.1: Gene Form
   

hdhA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

malI hdhA uidR anti-terminator terminator hdhAp hdhAp uidRp uidRp TSS_1898 TSS_1898

Gene      
Name: hdhA    Texpresso search in the literature
Synonym(s): ECK1614, EG10425, b1619, hsd, hsdH
Genome position(nucleotides): 1697273 <-- 1698040
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 47.27
External database links:  
ASAP:
 ABE-0005419
CGSC:
 32248
ECHOBASE:
 EB0420
ECOLIHUB:
 hdhA
OU-MICROARRAY:
 b1619
STRING:
 511145.b1619
COLOMBOS: hdhA


Product      
Name: 7-α-hydroxysteroid dehydrogenase
Synonym(s): HdhA, Hsd, HsdH
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 26.779
Isoelectric point: 5.041
Motif(s):
 
Type Positions Sequence Comment
18 -> 249 GAGAGIGKEIAITFATAGASVVVSDINADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
42 -> 43 DI UniProt: NAD.
68 -> 69 DI UniProt: NAD.
146 -> 146 S UniProt: Reduction of the catalytic efficiency by over 65%. No effect on the affinity for cholate and NAD..
159 -> 159 Y UniProt: Reduction of the catalytic efficiency by 87.7%. No effect on the affinity for cholate and NAD..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.1 - unassigned reversible reactions
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0032991 - protein-containing complex
molecular_function GO:0106281 - chenodeoxycholate 7-alpha-dehydrogenase (NAD+) activity
GO:0016491 - oxidoreductase activity
GO:0008709 - cholate 7-alpha-dehydrogenase activity
GO:0042802 - identical protein binding
GO:0051287 - NAD binding
biological_process GO:0006629 - lipid metabolic process
GO:0016042 - lipid catabolic process
GO:0008202 - steroid metabolic process
GO:0030573 - bile acid catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Kim KH., et al., 2021
[2] Lelong C., et al., 2007
[3] Tanaka N., et al., 1996
External database links:  
ALPHAFOLD:
 P0AET8
DIP:
 DIP-9875N
ECOCYC:
 7-ALPHA-HYDROXYSTEROID-DEH-MONOMER
ECOLIWIKI:
 b1619
INTERPRO:
 IPR020904
INTERPRO:
 IPR036291
INTERPRO:
 IPR002347
MODBASE:
 P0AET8
PDB:
 1AHH
PDB:
 1AHI
PDB:
 1FMC
PFAM:
 PF13561
PRIDE:
 P0AET8
PRINTS:
 PR00080
PRINTS:
 PR00081
PRODB:
 PRO_000022872
PROSITE:
 PS00061
REFSEQ:
 NP_416136
SMR:
 P0AET8
SWISSMODEL:
 P0AET8
UNIPROT:
 P0AET8


Operon      
Name: hdhA         
Operon arrangement:
Transcription unit        Promoter
hdhA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1898 1697074 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Kim KH., Lee CW., Pardhe BD., Hwang J., Do H., Lee YM., Lee JH., Oh TJ., 2021, Crystal structure of an apo 7α-hydroxysteroid dehydrogenase reveals key structural changes induced by substrate and co-factor binding., J Steroid Biochem Mol Biol 212:105945

 [2] Lelong C., Aguiluz K., Luche S., Kuhn L., Garin J., Rabilloud T., Geiselmann J., 2007, The Crl-RpoS regulon of Escherichia coli., Mol Cell Proteomics 6(4):648-59

 [3] Tanaka N., Nonaka T., Yoshimoto T., Tsuru D., Mitsui Y., 1996, Crystallization and preliminary X-ray crystallographic studies of 7alpha-hydroxysteroid dehydrogenase from Escherichia coli., Acta Crystallogr D Biol Crystallogr 52(Pt 1):215-7

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RegulonDB