RegulonDB

RegulonDB RegulonDB 10.10: Gene Form

hyaB gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	hyaB
Synonym(s):	ECK0964, EG10469, b0973
Genome position(nucleotides):	1033254 --> 1035047 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	56.52
External database links:

ASAP:	ABE-0003288
CGSC:	31784
ECHOBASE:	EB0464
ECOLIHUB:	hyaB
OU-MICROARRAY:	b0973
STRING:	511145.b0973
COLOMBOS:	hyaB

Shine dalgarno
Sequence:	gccaggcaatGAGGATaaacaggcATG

Product

Name:

hydrogenase 1 large subunit

Synonym(s):

HyaB, hydrogenase 1 alpha; subunit

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane,periplasmic space

Molecular weight:

66.253

Isoelectric point:

5.842

Motif(s):

Type	Positions	Sequence
	52 -> 52	M
	576 -> 576	C
	582 -> 582	H
	583 -> 583	V
	54 -> 582	RGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSPGYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAPRGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEILRTLHSFDPCLACSTH

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.6 - aerobic respiration
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
		1 - metabolism --> 1.4 - energy production/transport --> 1.4.1 - electron donors
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0030288 - outer membrane-bounded periplasmic space GO:0005886 - plasma membrane GO:0044569 - [Ni-Fe] hydrogenase complex
	molecular_function	GO:0005515 - protein binding GO:0009055 - electron transfer activity GO:0046872 - metal ion binding GO:0016491 - oxidoreductase activity GO:0016151 - nickel cation binding GO:0008901 - ferredoxin hydrogenase activity GO:0033748 - hydrogenase (acceptor) activity
	biological_process	GO:0006113 - fermentation GO:0009061 - anaerobic respiration GO:0019645 - anaerobic electron transport chain GO:0022900 - electron transport chain GO:1902421 - hydrogen metabolic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Evans RM., et al., 2018
[2] Evans RM., et al., 2021
[3] Przybyla AE., et al., 1992

External database links:

DIP:

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REFSEQ:

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Operon

Name:

hyaABCDEF

Operon arrangement:

Transcription unit	Promoter
hyaABCDEF	hyaAp
hyaABCDEF	hyaAp2

Transcriptional Regulation
	Display Regulation
Activated by:	YdeO, ArcA, AppY
Repressed by:	IscR, NarL, NarP, Fis

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Evans RM., Ash PA., Beaton SE., Brooke EJ., Vincent KA., Carr SB., Armstrong FA., 2018, Mechanistic Exploitation of a Self-Repairing, Blocked Proton Transfer Pathway in an O₂ -Tolerant [NiFe]-Hydrogenase., J Am Chem Soc 140(32):10208-10220
[2] Evans RM., Krahn N., Murphy BJ., Lee H., Armstrong FA., Soll D., 2021, Selective cysteine-to-selenocysteine changes in a [NiFe]-hydrogenase confirm a special position for catalysis and oxygen tolerance., Proc Natl Acad Sci U S A 118(13)
[3] Przybyla AE., Robbins J., Menon N., Peck HD., 1992, Structure-function relationships among the nickel-containing hydrogenases., FEMS Microbiol Rev 8(2):109-35

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