RegulonDB RegulonDB 10.9: Gene Form
   

metB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

metL metB metJ PhoP MetJ MetJ MetJ MetJ MetJ MetJ Fur TSS_4644 TSS_4644 TSS_4643 TSS_4643 metBp metBp TSS_4642 TSS_4642 metJp1 metJp1 metJp2 metJp2 metJp3 metJp3

Gene      
Name: metB    Texpresso search in the literature
Synonym(s): ECK3931, EG10582, b3939, met-1
Genome position(nucleotides): 4128672 --> 4129832 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.95
External database links:  
ASAP:
ABE-0012887
CGSC:
515
ECHOBASE:
EB0577
ECOLIHUB:
metB
MIM:
219500
OU-MICROARRAY:
b3939
STRING:
511145.b3939
COLOMBOS: metB


Shine dalgarno      
Sequence: acaaagcccaGGGAACttcatcacATG


Product      
Name: O-succinylhomoserine(thiol)-lyase / O-succinylhomoserine lyase
Synonym(s): MetB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 41.55
Isoelectric point: 6.44
Motif(s):
 
Type Positions Sequence
7 -> 380 TIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAHDYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYGGSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKICHLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDVVTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLYHPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLISHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGF

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.3 - amino acids
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.9 - methionine
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.30 - threonine catabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0102028 - cystathionine gamma-synthase activity (acts on O-phosphohomoserine)
GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0016846 - carbon-sulfur lyase activity
GO:0030170 - pyridoxal phosphate binding
GO:0003962 - cystathionine gamma-synthase activity
GO:0004123 - cystathionine gamma-lyase activity
GO:0042802 - identical protein binding
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009086 - methionine biosynthetic process
GO:0019343 - cysteine biosynthetic process via cystathionine
GO:0019346 - transsulfuration
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-10192N
ECOCYC:
O-SUCCHOMOSERLYASE-MONOMER
ECOLIWIKI:
b3939
INTERPRO:
IPR015421
INTERPRO:
IPR015424
INTERPRO:
IPR015422
INTERPRO:
IPR011821
INTERPRO:
IPR000277
MODBASE:
P00935
PANTHER:
PTHR11808
PDB:
1CS1
PFAM:
PF01053
PRIDE:
P00935
PRODB:
PRO_000023209
PROSITE:
PS00868
REFSEQ:
NP_418374
SMR:
P00935
UNIPROT:
P00935


Operon      
Name: metBL         
Operon arrangement:
Transcription unit        Promoter
metBL


Transcriptional Regulation      
Display Regulation             
Activated by: PhoP, HypT
Repressed by: MetJ


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4642 4128635 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4643 4129260 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4644 4129519 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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