Gene
Name:	murG
Synonym(s):	ECK0091, EG10623, b0090
Genome position(nucleotides):	99644 --> 100711 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	56.09
External database links:

ASAP:	ABE-0000322
CGSC:	473
ECHOBASE:	EB0618
ECOLIHUB:	murG
OU-MICROARRAY:	b0090
STRING:	511145.b0090
COLOMBOS:	murG

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_169	97532	forward	nd	[RS-EPT-CBR]	[20]
promoter	TSS_170	97539	forward	nd	[RS-EPT-CBR]	[20]
promoter	TSS_171	97550	forward	nd	[RS-EPT-CBR]	[20]
promoter	TSS_172	100621	forward	nd	[RS-EPT-CBR]	[20]
promoter	TSS_173 (cluster)	102049	forward	nd	[RS-EPT-CBR]	[20]

Reference(s)
[1] Barbosa MD., Ross HO., Hillman MC., Meade RP., Kurilla MG., Pompliano DL., 2002, A multitarget assay for inhibitors of membrane-associated steps of peptidoglycan biosynthesis., Anal Biochem 306(1):17-22
[2] Branstrom AA., Midha S., Longley CB., Han K., Baizman ER., Axelrod HR., 2000, Assay for identification of inhibitors for bacterial MraY translocase or MurG transferase., Anal Biochem 280(2):315-9
[3] Chandrakala B., Elias BC., Mehra U., Umapathy NS., Dwarakanath P., Balganesh TS., deSousa SM., 2001, Novel scintillation proximity assay for measuring membrane-associated steps of peptidoglycan biosynthesis in Escherichia coli., Antimicrob Agents Chemother 45(3):768-75
[4] Chen L., Men H., Ha S., Ye XY., Brunner L., Hu Y., Walker S., 2002, Intrinsic lipid preferences and kinetic mechanism of Escherichia coli MurG., Biochemistry 41(21):6824-33
[5] Crouvoisier M., Auger G., Blanot D., Mengin-Lecreulx D., 2007, Role of the amino acid invariants in the active site of MurG as evaluated by site-directed mutagenesis., Biochimie 89(12):1498-508
[6] Crouvoisier M., Mengin-Lecreulx D., van Heijenoort J., 1999, UDP-N-acetylglucosamine:N-acetylmuramoyl-(pentapeptide) pyrophosphoryl undecaprenol N-acetylglucosamine transferase from Escherichia coli: overproduction, solubilization, and purification., FEBS Lett 449(2-3):289-92
[7] Cudic P., Behenna DC., Yu MK., Kruger RG., Szewczuk LM., McCafferty DG., 2001, Synthesis of P(1)-Citronellyl-P(2)-alpha-D-pyranosyl pyrophosphates as potential substrates for the E. coli undecaprenyl-pyrophosphoryl-N-acetylglucoseaminyl transferase MurG., Bioorg Med Chem Lett 11(24):3107-10
[8] Hu Y., Helm JS., Chen L., Ginsberg C., Gross B., Kraybill B., Tiyanont K., Fang X., Wu T., Walker S., 2004, Identification of selective inhibitors for the glycosyltransferase MurG via high-throughput screening., Chem Biol 11(5):703-11
[9] Hyland SA., Anderson MS., 2003, A high-throughput solid-phase extraction assay capable of measuring diverse polyprenyl phosphate: sugar-1-phosphate transferases as exemplified by the WecA, MraY, and MurG proteins., Anal Biochem 317(2):156-65
[10] Ikeda M., Wachi M., Jung HK., Ishino F., Matsuhashi M., 1990, Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli., Nucleic Acids Res 18(13):4014
[11] Liu H., Ritter TK., Sadamoto R., Sears PS., Wu M., Wong CH., 2003, Acceptor specificity and inhibition of the bacterial cell-wall glycosyltransferase MurG., Chembiochem 4(7):603-9
[12] Mengin-Lecreulx D., Ayala J., Bouhss A., van Heijenoort J., Parquet C., Hara H., 1998, Contribution of the Pmra promoter to expression of genes in the Escherichia coli mra cluster of cell envelope biosynthesis and cell division genes., J Bacteriol 180(17):4406-12
[13] Mengin-Lecreulx D., Parquet C., Desviat LR., Pla J., Flouret B., Ayala JA., van Heijenoort J., 1989, Organization of the murE-murG region of Escherichia coli: identification of the murD gene encoding the D-glutamic-acid-adding enzyme., J Bacteriol 171(11):6126-34
[14] Mengin-Lecreulx D., Texier L., van Heijenoort J., 1990, Nucleotide sequence of the cell-envelope murG gene of Escherichia coli., Nucleic Acids Res 18(9):2810
[15] Ravishankar S., Kumar VP., Chandrakala B., Jha RK., Solapure SM., de Sousa SM., 2005, Scintillation proximity assay for inhibitors of Escherichia coli MurG and, optionally, MraY., Antimicrob Agents Chemother 49(4):1410-8
[16] Salmond GP., Lutkenhaus JF., Donachie WD., 1980, Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell envelope gene murG., J Bacteriol 144(1):438-40
[17] Wrabl JO., Grishin NV., 2001, Homology between O-linked GlcNAc transferases and proteins of the glycogen phosphorylase superfamily., J Mol Biol 314(3):365-74
[18] Zawadzke LE., Wu P., Cook L., Fan L., Casperson M., Kishnani M., Calambur D., Hofstead SJ., Padmanabha R., 2003, Targeting the MraY and MurG bacterial enzymes for antimicrobial therapeutic intervention., Anal Biochem 314(2):243-52
[19] van den Brink-van der Laan E., Boots JW., Spelbrink RE., Kool GM., Breukink E., Killian JA., de Kruijff B., 2003, Membrane interaction of the glycosyltransferase MurG: a special role for cardiolipin., J Bacteriol 185(13):3773-9
[20] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Transcriptional Regulation
	Display Regulation
Repressed by:	MraZ, LexA, PdhR