RegulonDB RegulonDB 11.2: Gene Form
   

narJ gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

narG narH narJ narI ychS Cis-reg Cis-reg terminator TSS_1555 TSS_1555

Gene      
Name: narJ    Texpresso search in the literature
Synonym(s): ECK1220, EG10641, b1226
Genome position(nucleotides): 1285139 --> 1285849
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 56.4
External database links:  
ASAP:
 ABE-0004123
CGSC:
 18115
ECHOBASE:
 EB0635
ECOLIHUB:
 narJ
OU-MICROARRAY:
 b1226
STRING:
 511145.b1226
COLOMBOS: narJ


Product      
Name: nitrate reductase 1 molybdenum cofactor assembly chaperone
Synonym(s): NarJ, molybdenum cofactor assembly chaperone subunit (delta; subunit) of nitrate reductase 1
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 26.449
Isoelectric point: 4.52
Motif(s):
 
Type Positions Sequence Comment
47 -> 155 LRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHGLQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYA

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0051082 - unfolded protein binding
GO:0016530 - metallochaperone activity
biological_process GO:0051131 - chaperone-mediated protein complex assembly
GO:0042128 - nitrate assimilation
Note(s): Note(s): ...[more].
Evidence: [EXP] Inferred from experiment
External database links:  
ALPHAFOLD:
 P0AF26
DIP:
 DIP-35943N
ECOCYC:
 NARJ-MONOMER
ECOLIWIKI:
 b1226
INTERPRO:
 IPR042291
INTERPRO:
 IPR003765
INTERPRO:
 IPR036411
INTERPRO:
 IPR042290
INTERPRO:
 IPR020945
MINT:
 P0AF26
MODBASE:
 P0AF26
PANTHER:
 PTHR43680
PFAM:
 PF02613
PRIDE:
 P0AF26
PRODB:
 PRO_000023354
REFSEQ:
 NP_415744
SMR:
 P0AF26
UNIPROT:
 P0AF26


Operon      
Name: narGHJI         
Operon arrangement:
Transcription unit        Promoter
narGHJI


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, IHF, RstA, NarL
Repressed by: Fis


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1555 1280972 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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