|
|
15 -> 37
|
VALIVLLSTAIGLAGMAVSGWLV
|
UniProt: Helical.
|
|
|
35 -> 129
|
WLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGLQDYWRNELIPALMRAQNRETVSADVSQ
|
|
|
|
39 -> 65
|
GVQGSAHAINKAGSLRMQSYRLLAAVP
|
conserved periplasmic-box
|
|
|
42 -> 52
|
GSAHAINKAGS
|
UniProt: In Ref. 1 and 5..
|
|
|
45 -> 45
|
H
|
H → E: nitrite-insensitive phenotype
|
|
|
46 -> 46
|
A
|
A → T: class I; 'locked on' phenotype; results in constitutive activation or repression of reporter gene expression even in the absence of nitrate or nitrite
|
|
|
49 -> 49
|
K
|
K → I; significantly impaired ability to discriminate between nitrate and nitrite
|
|
|
51 -> 51
|
G
|
G → R: nitrate-insensitive phenotype
|
|
|
54 -> 54
|
R
|
R → S/K: ligand insensitive phenotype
|
|
|
59 -> 59
|
R
|
R → K: nitrite-insensitive phenotype
|
|
|
152 -> 174
|
VVLVHRVMAVFMALLLVFTIIWL
|
UniProt: Helical.
|
|
|
173 -> 223
|
WLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAEL
|
|
|
|
175 -> 254
|
RARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQA
|
|
|
|
176 -> 228
|
ARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYA
|
UniProt: HAMP.
|
|
|
205 -> 205
|
G
|
G → R: confers nitrate independent expression of narGHJI and fdnG-lacZ; recessive to narX+; see also |CITS: [2254274]|
|
|
|
208 -> 208
|
E
|
E → K: confers nitrate independent expression of narGHJI and fdnG-lacZ; recessive to narX+; see also |CITS: [2254274]|
|
|
|
212 -> 212
|
L
|
L → A: heptad position 'd'; results in a 'constitutive' or 'elevated basal' phenotype
|
|
|
216 -> 216
|
L
|
L → A: heptad position 'a'; results in a 'constitutive' or 'elevated basal' phenotype
|
|
|
218 -> 257
|
NMSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRR
|
implicated (along with HAMP domain) in conducting ligand responsive motions from the transmembrane signaling module to the central module
|
|
|
219 -> 219
|
M
|
M → I: confers nitrate independent expression of narGHJI and fdnG-lacZ; recessive to narX+
|
|
|
223 -> 223
|
L
|
L → A: results in a 'constitutive' or 'elevated basal' phenotype
|
|
|
224 -> 224
|
A
|
A → V: confers nitrate independent expression of narGHJI and fdnG-lacZ; recessive to narX+
|
|
|
227 -> 227
|
Y
|
Y → A: heptad position 'a'; results in a 'constitutive' or 'locked-on' phenotype
|
|
|
230 -> 230
|
L
|
L → A: heptad position 'd'; results in a 'constitutive' or 'elevated basal' phenotype
|
|
|
233 -> 233
|
R
|
R → E: results in an 'impaired induction' phenotype
|
|
|
236 -> 238
|
EKT
|
|
|
|
237 -> 237
|
K
|
K → E: heptad position 'd'; results in an 'elevated basal' phenotype
|
|
|
238 -> 238
|
T
|
T → F: results in an 'elevated basal' phenotype
|
|
|
241 -> 241
|
L
|
L → A: heptad position 'a'; results in an 'impaired induction' phenotype
|
|
|
251 -> 251
|
L
|
L → A: heptad position 'd'; results in a 'reversed response' phenotype
|
|
|
252 -> 252
|
W
|
W → R: results in a 'constitutive' or 'locked-on' phenotype
|
|
|
374 -> 374
|
A
|
UniProt: In Ref. 6; AAA24198..
|
|
|
390 -> 453
|
ERATIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLTTFR
|
|
|
|
393 -> 587
|
TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGDCRVRRRESGGTEVVVTFIPEK
|
UniProt: Histidine kinase.
|
|
|
397 -> 405
|
ELHDSIAQS
|
|
|
|
399 -> 399
|
H
|
H → E: loss of autokinase activity in vitro, retains some NarL-phosphate phosphatase activity
|
|
|
400 -> 400
|
D
|
D → A/S/V: loss of transmitter phosphatase function
|
|
|
404 -> 404
|
Q
|
Q → A/E: loss of transmitter phosphatase activity
|
|
|
410 -> 410
|
K
|
K → E: confers 'NarP-blind' phenotype (see |CITS: [19968795]| for further detail)
|
|
|
411 -> 411
|
M
|
M → T: confers 'NarP-blind' phenotype (see |CITS: [19968795]| for further detail)
|
|
|
434 -> 457
|
IRNELNASWAQLRELLTTFRLQLT
|
|
|
|
442 -> 442
|
W
|
W → R: confers 'NarP-blind' phenotype (see |CITS: [19968795]| for further detail)
|
|
|
452 -> 452
|
F
|
F → Y: confers 'NarP-blind' phenotype (see |CITS: [19968795]| for further detail)
|
|
|
496 -> 583
|
AIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGDCRVRRRESGGTEVVVTF
|
|
|
|
509 -> 509
|
N
|
N → D: loss of autokinase activity in vitro retains ability to dephosphorylate NarL-phosphate; unable to activate or repress frdA-lacZ expression in response to nitrate
|
|
|
513 -> 513
|
H
|
H → Q: loss of autokinase activity in vitro retains ability to dephosphorylate NarL-phosphate; unable to activate or repress frdA-lacZ expression in response to nitrate
|
