RegulonDB RegulonDB 10.8: Gene Form
   

nadE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cho nadE osmE spy3\' ves LexA Fis Fis IHF Fis CyaR chop chop TSS_2052 TSS_2052 TSS_2051 TSS_2051 TSS_2050 TSS_2050 nadEp nadEp osmEp osmEp TSS_2048 (cluster) TSS_2048 (cluster) TSS_2047 (cluster) TSS_2047 (cluster)

Gene      
Name: nadE    Texpresso search in the literature
Synonym(s): ECK1738, EG10663, b1740, efg, ntrL
Genome position(nucleotides): 1822458 --> 1823285 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.52


Product      
Name: NH3-dependent NAD+ synthetase
Synonym(s): Efg, NadE, NtrL
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 30.637
Isoelectric point: 5.232
Motif(s):
 
Type Positions Sequence
23 -> 265 IRRSVDFLKSYLQTYPFIKSLVLGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGTDHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDRPSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPP
46 -> 53 GISGGQDS
260 -> 261 HK
13 -> 31 AKPQINAEEEIRRSVDFLK
170 -> 180 FFTKYGDGGTD

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.7 - nicotinamide adenine dinucleotide
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0003952 - NAD+ synthase (glutamine-hydrolyzing) activity
GO:0004359 - glutaminase activity
GO:0008795 - NAD+ synthase activity
GO:0000287 - magnesium ion binding
GO:0042803 - protein homodimerization activity
biological_process GO:0006974 - cellular response to DNA damage stimulus
GO:0009435 - NAD biosynthetic process
GO:0034355 - NAD salvage
GO:0034628 - 'de novo' NAD biosynthetic process from aspartate
Note(s): Note(s): ...[more].
Reference(s): [1] Gutierrez C., et al., 1995
[2] Song HS., et al., 2017
[3] Zhou Y., et al., 2011
External database links:  
DIP:
 DIP-10295N
ECOCYC:
 NAD-SYNTH-MONOMER
ECOLIWIKI:
 B1740
INTERPRO:
 IPR022926
INTERPRO:
 IPR022310
INTERPRO:
 IPR014729
INTERPRO:
 IPR003694
MINT:
 MINT-1232772
MODBASE:
 P18843
PANTHER:
 PTHR23090
PDB:
 1WXI
PDB:
 1WXE
PDB:
 1WXH
PDB:
 1WXF
PDB:
 1WXG
PFAM:
 PF02540
PRIDE:
 P18843
PRODB:
 PRO_000023335
REFSEQ:
 NP_416254
SMR:
 P18843
UNIPROT:
 P18843


Operon      
Name: nadE         
Operon arrangement:
Transcription unit        Promoter
nadE


Regulation by small RNA    
  Display Regulation
small RNA cyaR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2047 (cluster) 1822275 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [4]
  promoter TSS_2048 (cluster) 1822278 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [4]
  promoter TSS_2050 1822428 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2051 1822436 forward nd [RS-EPT-CBR] [4]
  promoter TSS_2052 1822732 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Gutierrez C., Gordia S., Bonnassie S., 1995, Characterization of the osmotically inducible gene osmE of Escherichia coli K-12., Mol Microbiol 16(3):553-63

 [2] Song HS., Jeon JM., Kim HJ., Bhatia SK., Sathiyanarayanan G., Kim J., Won Hong J., Gi Hong Y., Young Choi K., Kim YG., Kim W., Yang YH., 2017, Increase in furfural tolerance by combinatorial overexpression of NAD salvage pathway enzymes in engineered isobutanol-producing E. coli., Bioresour Technol 245(Pt B):1430-1435

 [3] Zhou Y., Wang L., Yang F., Lin X., Zhang S., Zhao ZK., 2011, Determining the extremes of the cellular NAD(H) level by using an Escherichia coli NAD(+)-auxotrophic mutant., Appl Environ Microbiol 77(17):6133-40

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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