RegulonDB RegulonDB 10.9: Gene Form
   

pfkA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

sbp pfkA fieF Cra anti-anti-terminator terminator anti-terminator anti-anti-terminator terminator TSS_4611 TSS_4611 TSS_4610 TSS_4610 TSS_4609 TSS_4609 TSS_4608 (cluster) TSS_4608 (cluster) TSS_4607 TSS_4607 TSS_4606 (cluster) TSS_4606 (cluster) TSS_4605 (cluster) TSS_4605 (cluster) TSS_4604 (cluster) TSS_4604 (cluster) pfkAp1 pfkAp1 TSS_4603 (cluster) TSS_4603 (cluster) pfkAp2 pfkAp2 TSS_4601 (cluster) TSS_4601 (cluster) TSS_4600 (cluster) TSS_4600 (cluster)

Gene      
Name: pfkA    Texpresso search in the literature
Synonym(s): ECK3908, EG10699, b3916
Genome position(nucleotides): 4107552 --> 4108514 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.48
External database links:  
ASAP:
ABE-0012789
CGSC:
413
ECHOBASE:
EB0693
ECOLIHUB:
pfkA
OU-MICROARRAY:
b3916
STRING:
511145.b3916
COLOMBOS: pfkA


Product      
Name: 6-phosphofructokinase 1
Synonym(s): 6-phosphofructokinase I, PfkA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 34.842
Isoelectric point: 5.448
Motif(s):
 
Type Positions Sequence
73 -> 74 RF
170 -> 172 MGR
126 -> 128 TID
250 -> 253 HIQR
172 -> 172 R

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005945 - 6-phosphofructokinase complex
molecular_function GO:0070095 - fructose-6-phosphate binding
GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0003872 - 6-phosphofructokinase activity
GO:0008443 - phosphofructokinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0003951 - NAD+ kinase activity
GO:0048029 - monosaccharide binding
GO:0032553 - ribonucleotide binding
GO:0000287 - magnesium ion binding
GO:0016208 - AMP binding
GO:0042802 - identical protein binding
GO:0019003 - GDP binding
biological_process GO:0061621 - canonical glycolysis
GO:0008152 - metabolic process
GO:0006007 - glucose catabolic process
GO:0006096 - glycolytic process
GO:0044275 - cellular carbohydrate catabolic process
GO:0016310 - phosphorylation
GO:0006002 - fructose 6-phosphate metabolic process
GO:0046835 - carbohydrate phosphorylation
GO:0051289 - protein homotetramerization
GO:0030388 - fructose 1,6-bisphosphate metabolic process
External database links:  
DIP:
DIP-35841N
ECOCYC:
6PFK-1-MONOMER
ECOLIWIKI:
b3916
INTERPRO:
IPR012828
INTERPRO:
IPR035966
INTERPRO:
IPR022953
INTERPRO:
IPR015912
INTERPRO:
IPR012003
INTERPRO:
IPR000023
MODBASE:
P0A796
PDB:
2PFK
PDB:
1PFK
PFAM:
PF00365
PRIDE:
P0A796
PRINTS:
PR00476
PRODB:
PRO_000023514
PROSITE:
PS00433
REFSEQ:
NP_418351
SMR:
P0A796
UNIPROT:
P0A796


Operon      
Name: pfkA         
Operon arrangement:
Transcription unit        Promoter
pfkA
pfkA
pfkA
pfkA


Transcriptional Regulation      
Display Regulation             
Repressed by: Cra


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4600 (cluster) 4107207 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4601 (cluster) 4107419 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4603 (cluster) 4107519 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4604 (cluster) 4107525 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4605 (cluster) 4107530 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4606 (cluster) 4107538 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4607 4107556 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4608 (cluster) 4107822 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4609 4107877 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4610 4108126 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4611 4108805 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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