RegulonDB RegulonDB 10.8: Gene Form
   

pyrG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pyrG eno mazG Cra Cra anti-anti-terminator anti-terminator terminator pyrGp1 pyrGp1 TSS_3083 (cluster) TSS_3083 (cluster) pyrGp2 pyrGp2 TSS_3082 (cluster) TSS_3082 (cluster) pyrGp pyrGp TSS_3081 TSS_3081 TSS_3080 TSS_3080 TSS_3079 TSS_3079 TSS_3078 TSS_3078 TSS_3077 TSS_3077 TSS_3076 TSS_3076 enop3 enop3 enop2 enop2 enop1 enop1 TSS_3075 TSS_3075 TSS_3074 (cluster) TSS_3074 (cluster) enop7 enop7 TSS_3073 (cluster) TSS_3073 (cluster) enop6 enop6 TSS_3072 (cluster) TSS_3072 (cluster) enop4 enop4 TSS_3070 TSS_3070 TSS_3069 TSS_3069 TSS_3068 TSS_3068 TSS_3067 TSS_3067 TSS_3066 TSS_3066 TSS_3065 TSS_3065 TSS_3064 (cluster) TSS_3064 (cluster) TSS_3063 TSS_3063 TSS_3062 TSS_3062 TSS_3061 TSS_3061 TSS_3060 TSS_3060 TSS_3059 TSS_3059 TSS_3058 (cluster) TSS_3058 (cluster) TSS_3057 (cluster) TSS_3057 (cluster) TSS_3056 TSS_3056 TSS_3055 TSS_3055

Gene      
Name: pyrG    Texpresso search in the literature
Synonym(s): ECK2774, EG10810, b2780
Genome position(nucleotides): 2908029 <-- 2909666 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.89
External database links:  
ASAP:
ABE-0009112
CGSC:
325
ECHOBASE:
EB0803
MIM:
615897
OU-MICROARRAY:
b2780
PortEco:
pyrG
STRING:
511145.b2780
COLOMBOS: pyrG


Product      
Name: CTP synthetase
Synonym(s): CTP synthase, PyrG
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 60.374
Isoelectric point: 5.779
Motif(s):
 
Type Positions Sequence
301 -> 535 LPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVETRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARHVANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVDDSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPWFVACQFHPEFTSTPRDGHPLFAGFVKA
147 -> 149 DIE
349 -> 349 V
380 -> 383 LGMQ
476 -> 476 M

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.4 - pyrimidine ribonucleotide biosynthesis
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0032991 - protein-containing complex
molecular_function GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0003883 - CTP synthase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0000287 - magnesium ion binding
GO:0042802 - identical protein binding
biological_process GO:0006221 - pyrimidine nucleotide biosynthetic process
GO:0006541 - glutamine metabolic process
GO:0006241 - CTP biosynthetic process
GO:0019856 - pyrimidine nucleobase biosynthetic process
GO:0051289 - protein homotetramerization
GO:0044210 - 'de novo' CTP biosynthetic process
External database links:  
DIP:
DIP-10628N
ECOCYC:
CTPSYN-MONOMER
ECOLIWIKI:
b2780
INTERPRO:
IPR017456
INTERPRO:
IPR004468
INTERPRO:
IPR017926
INTERPRO:
IPR029062
INTERPRO:
IPR033828
INTERPRO:
IPR027417
PANTHER:
PTHR11550
PDB:
5U6R
PDB:
5U3C
PDB:
5U05
PDB:
5TKV
PDB:
2AD5
PDB:
1S1M
PFAM:
PF06418
PFAM:
PF00117
PRIDE:
P0A7E5
PRODB:
PRO_000023662
PROSITE:
PS51273
REFSEQ:
NP_417260
SMR:
P0A7E5
UNIPROT:
P0A7E5


Operon      
Name: pyrG-eno         
Operon arrangement:
Transcription unit        Promoter
eno
eno
eno
eno
eno
eno
pyrG-eno
pyrG-eno
pyrG-eno


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3055 2907117 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3056 2907189 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3057 (cluster) 2907196 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_3058 (cluster) 2907241 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_3059 2907580 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3060 2907627 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3061 2907633 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3062 2907661 forward nd [RS-EPT-CBR] [1]
  promoter TSS_3063 2907662 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3064 (cluster) 2907665 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_3065 2907682 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3066 2907694 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3067 2907716 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3068 2907923 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3069 2907932 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3070 2907956 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3072 (cluster) 2907975 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_3073 (cluster) 2908021 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_3074 (cluster) 2908226 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_3075 2908550 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3076 2908879 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3077 2908881 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3078 2909694 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3079 2909704 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3080 2909706 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3081 2909711 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_3082 (cluster) 2909716 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_3083 (cluster) 2909795 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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