RegulonDB RegulonDB 11.1: Gene Form
   

recN gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

recN nadK bamE NsrR CpxR DnaA LexA DnaA LexA CRP TSS_2970 TSS_2970 TSS_2969 TSS_2969 TSS_2968 TSS_2968 TSS_2967 TSS_2967 TSS_2966 TSS_2966 TSS_2965 TSS_2965 bamEp bamEp TSS_2964 TSS_2964 recNp recNp

Gene      
Name: recN    Texpresso search in the literature
Synonym(s): ECK2612, EG10831, b2616, radB
Genome position(nucleotides): 2751795 --> 2753456
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.81
External database links:  
ASAP:
 ABE-0008611
CGSC:
 10872
ECHOBASE:
 EB0824
ECOLIHUB:
 recN
OU-MICROARRAY:
 b2616
STRING:
 511145.b2616
COLOMBOS: recN


Product      
Name: DNA repair protein RecN
Synonym(s): RadB, RecN
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,bacterial nucleoid
Molecular weight: 61.396
Isoelectric point: 5.347
Motif(s):
 
Type Positions Sequence Comment
1 -> 511 MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGLCLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGRSRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMTARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLANSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQIAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQQLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQFTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARKMETPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKETDG
29 -> 36 GETGAGKS UniProt: ATP.
35 -> 35 K K → A: loss of function mutation; is recruited to sites of DNA damage but may not be properly released
59 -> 60 AA UniProt: In Ref. 1; CAA68435..
76 -> 76 A UniProt: In Ref. 1; CAA68435..

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.1 - DNA related --> 2.1.3 - DNA recombination
  2 - information transfer --> 2.1 - DNA related --> 2.1.4 - DNA repair
  5 - cell processes --> 5.8 - SOS response
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0043590 - bacterial nucleoid
molecular_function GO:0005515 - protein binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
biological_process GO:0010165 - response to X-ray
GO:0036298 - recombinational interstrand cross-link repair
GO:0006281 - DNA repair
GO:0006310 - DNA recombination
GO:0006974 - cellular response to DNA damage stimulus
GO:0009432 - SOS response
GO:0009314 - response to radiation
GO:0009411 - response to UV
GO:0000725 - recombinational repair
GO:0006302 - double-strand break repair
GO:0010212 - response to ionizing radiation
GO:0000724 - double-strand break repair via homologous recombination
Note(s): Note(s): ...[more].
Evidence: [EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Almeida E., et al., 2010
[2] Berger P., et al., 2019
[3] Brena-Valle M., et al., 1998
[4] Courcelle J., et al., 2001
[5] Cupido M., et al., 1985
[6] Dunman PM., et al., 2000
[7] Goswami M., et al., 2018
[8] Keyamura K., et al., 2019
[9] Keyamura K., et al., 2013
[10] Klimova AN., et al., 2020
[11] Lloyd RG., et al., 1991
[12] Lloyd RG., et al., 1983
[13] Odsbu I., et al., 2014
[14] Picksley SM., et al., 1984
[15] Rostas K., et al., 1987
[16] Sargentini NJ., et al., 1988
[17] Sargentini NJ., et al., 1986
[18] Sargentini NJ., et al., 1983
[19] Takahashi NK., et al., 1993
[20] Vickridge E., et al., 2017
[21] Warr AR., et al., 2019
External database links:  
ALPHAFOLD:
 P05824
DIP:
 DIP-10655N
ECOCYC:
 EG10831-MONOMER
ECOLIWIKI:
 b2616
INTERPRO:
 IPR004604
INTERPRO:
 IPR003395
INTERPRO:
 IPR027417
MODBASE:
 P05824
PANTHER:
 PTHR11059
PFAM:
 PF02463
PRIDE:
 P05824
PRODB:
 PRO_000023704
REFSEQ:
 YP_026172
SMR:
 P05824
UNIPROT:
 P05824


Operon      
Name: recN         
Operon arrangement:
Transcription unit        Promoter
recN


Transcriptional Regulation      
Display Regulation             
Repressed by: DnaA, LexA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2964 2752658 reverse nd [RS-EPT-CBR] [22]
  promoter TSS_2965 2753488 forward nd [RS-EPT-CBR] [22]
  promoter TSS_2966 2753555 forward nd [RS-EPT-CBR] [22]
  promoter TSS_2967 2753588 forward nd [RS-EPT-CBR] [22]
  promoter TSS_2968 2753594 forward nd [RS-EPT-CBR] [22]
  promoter TSS_2969 2753599 forward nd [RS-EPT-CBR] [22]
  promoter TSS_2970 2753605 forward nd [RS-EPT-CBR] [22]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Almeida E., Fuentes JL., Cuetara E., Prieto E., Llagostera M., 2010, Amifostine protection against induced DNA damage in gamma-irradiated Escherichia coli cells depend on recN DNA repair gene product activity., Environ Toxicol 25(2):130-6

 [2] Berger P., Kouzel IU., Berger M., Haarmann N., Dobrindt U., Koudelka GB., Mellmann A., 2019, Carriage of Shiga toxin phage profoundly affects Escherichia coli gene expression and carbon source utilization., BMC Genomics 20(1):504

 [3] Brena-Valle M., Serment-Guerrero J., 1998, SOS induction by gamma-radiation in Escherichia coli strains defective in repair and/or recombination mechanisms., Mutagenesis 13(6):637-41

 [4] Courcelle J., Khodursky A., Peter B., Brown PO., Hanawalt PC., 2001, Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli., Genetics 158(1):41-64

 [5] Cupido M., Bridges BA., 1985, Uvr-independent repair of 8-methoxypsoralen crosslinks in Escherichia coli: evidence for a recombinational process., Mutat Res 146(2):135-41

 [6] Dunman PM., Ren L., Rahman MS., Palejwala VA., Murphy HS., Volkert MR., Humayun MZ., 2000, Escherichia coli cells defective for the recN gene display constitutive elevation of mutagenesis at 3,N(4)-ethenocytosine via an SOS-induced mechanism., Mol Microbiol 37(3):680-6

 [7] Goswami M., Narayana Rao AVSS., 2018, Transcriptome Profiling Reveals Interplay of Multifaceted Stress Response in Escherichia coli on Exposure to Glutathione and Ciprofloxacin., mSystems 3(1)

 [8] Keyamura K., Hishida T., 2019, Topological DNA-binding of structural maintenance of chromosomes-like RecN promotes DNA double-strand break repair in Escherichia coli., Commun Biol 2:413

 [9] Keyamura K., Sakaguchi C., Kubota Y., Niki H., Hishida T., 2013, RecA protein recruits structural maintenance of chromosomes (SMC)-like RecN protein to DNA double-strand breaks., J Biol Chem 288(41):29229-37

 [10] Klimova AN., Sandler SJ., 2020, An Epistasis Analysis of recA and recN in Escherichia coli K-12., Genetics 216(2):381-393

 [11] Lloyd RG., Buckman C., 1991, Overlapping functions of recD, recJ and recN provide evidence of three epistatic groups of genes in Escherichia coli recombination and DNA repair., Biochimie 73(2-3):313-20

 [12] Lloyd RG., Picksley SM., Prescott C., 1983, Inducible expression of a gene specific to the RecF pathway for recombination in Escherichia coli K12., Mol Gen Genet 190(1):162-7

 [13] Odsbu I., Skarstad K., 2014, DNA compaction in the early part of the SOS response is dependent on RecN and RecA., Microbiology 160(Pt 5):872-82

 [14] Picksley SM., Attfield PV., Lloyd RG., 1984, Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN product., Mol Gen Genet 195(1-2):267-74

 [15] Rostas K., Morton SJ., Picksley SM., Lloyd RG., 1987, Nucleotide sequence and LexA regulation of the Escherichia coli recN gene., Nucleic Acids Res 15(13):5041-9

 [16] Sargentini NJ., Smith KC., 1988, Genetic and phenotypic analyses indicating occurrence of the recN262 and radB101 mutations at the same locus in Escherichia coli., J Bacteriol 170(5):2392-4

 [17] Sargentini NJ., Smith KC., 1986, Quantitation of the involvement of the recA, recB, recC, recF, recJ, recN, lexA, radA, radB, uvrD, and umuC genes in the repair of X-ray-induced DNA double-strand breaks in Escherichia coli., Radiat Res 107(1):58-72

 [18] Sargentini NJ., Smith KC., 1983, Characterization of an Escherichia coli mutant (radB101) sensitive to gamma and uv radiation, and methyl methanesulfonate., Radiat Res 93(3):461-78

 [19] Takahashi NK., Kusano K., Yokochi T., Kitamura Y., Yoshikura H., Kobayashi I., 1993, Genetic analysis of double-strand break repair in Escherichia coli., J Bacteriol 175(16):5176-85

 [20] Vickridge E., Planchenault C., Cockram C., Junceda IG., Espeli O., 2017, Management of E. coli sister chromatid cohesion in response to genotoxic stress., Nat Commun 8:14618

 [21] Warr AR., Klimova AN., Nwaobasi AN., Sandler SJ., 2019, Protease-deficient SOS constitutive cells have RecN-dependent cell division phenotypes., Mol Microbiol 111(2):405-422

 [22] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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