RegulonDB

Gene
Name:	sfsA
Synonym(s):	ECK0145, EG10949, b0146, sfs, sfs1
Genome position(nucleotides):	160782 <-- 161486
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.36
External database links:

ASAP:	ABE-0000503
CGSC:	30524
ECHOBASE:	EB0942
ECOLIHUB:	sfsA
OU-MICROARRAY:	b0146
STRING:	511145.b0146
COLOMBOS:	sfsA

Product

Name:

sugar fermentation stimulation protein A

Synonym(s):

Sfs, SfsA, putative DNA-binding transcriptional regulator of maltose metabolism

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

26.229

Isoelectric point:

7.029

Motif(s):

Type	Positions	Sequence	Comment
	13 -> 80	IQRYKRFLADVITPDGRELTLHCPNTGAMTGCATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICV
	85 -> 222	ANRLTKEAILNESISELSGYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKHLRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAEISAE
	201 -> 220	LLSEAQQRGVEILAYKAEIS	UniProt: H-T-H motif.

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
		3 - regulation --> 3.1 - type of regulation --> 3.1.4 - unknown
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding
	biological_process	GO:0009891 - positive regulation of biosynthetic process

Note(s):

Note(s): ...[more].

Evidence:

[COMP-AINF-FN-FROM-SEQ] Automated inference of function from sequence
[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Kawamukai M., et al., 1991
[2] Perez-Rueda E., et al., 2004

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PDB:

PFAM:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

sfsA-dksA-gluQ

Operon arrangement:

Transcription unit	Promoter
dksA-yadB	dksAp2
dksA-yadB	dksAp1
dksA-gluQ	dksAp3
sfsA-dksA	sfsAp

Transcriptional Regulation
	Display Regulation
Activated by:	CRP

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_274	160641	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_275 (cluster)	160652	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_277 (cluster)	160761	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_278	160873	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_279	160884	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_280	161071	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_281 (cluster)	161079	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_282	161142	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_283	161187	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_284 (cluster)	161195	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_285	161199	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_286	161665	forward	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Kawamukai M., Utsumi R., Takeda K., Higashi A., Matsuda H., Choi YL., Komano T., 1991, Nucleotide sequence and characterization of the sfs1 gene: sfs1 is involved in CRP*-dependent mal gene expression in Escherichia coli., J Bacteriol 173(8):2644-8
[2] Perez-Rueda E., Collado-Vides J., Segovia L., 2004, Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea., Comput Biol Chem 28(5-6):341-50
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.