RegulonDB

Gene
Name:	ppsR
Synonym(s):	ECK1701, EG11132, b1703, ydiA
Genome position(nucleotides):	1787445 --> 1788278
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.32
External database links:

ASAP:	ABE-0005682
ECHOBASE:	EB1122
ECOLIHUB:	ppsR
OU-MICROARRAY:	b1703
STRING:	511145.b1703
COLOMBOS:	ppsR

Product

Name:

phosphoenolpyruvate synthetase regulatory protein

Synonym(s):

PEP synthetase regulatory protein, PpsR, YdiA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

31.211

Isoelectric point:

6.402

Motif(s):

Type	Positions	Sequence	Comment
	9 -> 267	VFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVENESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQEMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSRCGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIREERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKIL
	157 -> 164	GVSRCGKT	UniProt: ADP.

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.3 - protein related --> 2.3.3 - posttranslational modification
		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.2 - covalent modification, demodification, maturation
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0032991 - protein-containing complex
	molecular_function	GO:0005515 - protein binding GO:0030234 - enzyme regulator activity GO:0016740 - transferase activity GO:0016772 - transferase activity, transferring phosphorus-containing groups GO:0016301 - kinase activity GO:0016776 - phosphotransferase activity, phosphate group as acceptor GO:0004674 - protein serine/threonine kinase activity GO:0000166 - nucleotide binding GO:0005524 - ATP binding GO:0043531 - ADP binding
	biological_process	GO:0033673 - negative regulation of kinase activity GO:0006470 - protein dephosphorylation GO:0006468 - protein phosphorylation GO:0016310 - phosphorylation GO:0033674 - positive regulation of kinase activity GO:0051289 - protein homotetramerization

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

ppsR

Operon arrangement:

Transcription unit	Promoter
ppsR

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	ppsRp1	1787339	forward	nd	[COMP-AINF]	[1]
promoter	ppsRp8	1787351	forward	nd	[COMP-AINF]	[1]
promoter	ppsRp6	1787383	forward	nd	[COMP-AINF]	[1]
promoter	TSS_1991	1787415	forward	nd	[RS-EPT-CBR]	[2]
promoter	TSS_1992	1788321	forward	nd	[RS-EPT-CBR]	[2]
promoter	TSS_1994 (cluster)	1788419	forward	nd	[RS-EPT-CBR]	[2]
promoter	TSS_1995	1789271	forward	nd	[RS-EPT-CBR]	[2]

Evidence
[COMP-AINF] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.