RegulonDB RegulonDB 10.9: Gene Form
   

coaD gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

waaA mutM coaD TSS_4212 TSS_4212

Gene      
Name: coaD    Texpresso search in the literature
Synonym(s): ECK3624, EG11190, b3634, kdtB, yicA
Genome position(nucleotides): 3809825 --> 3810304 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.71
External database links:  
ASAP:
ABE-0011875
CGSC:
33900
ECHOBASE:
EB1176
ECOLIHUB:
coaD
OU-MICROARRAY:
b3634
STRING:
511145.b3634
COLOMBOS: coaD


Product      
Name: pantetheine-phosphate adenylyltransferase
Synonym(s): CoaD, KdtB, PPAT, YicA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 17.837
Isoelectric point: 7.044
Motif(s):
 
Type Positions Sequence
89 -> 91 GLR
124 -> 127 WSFI
7 -> 11 YPGTF
6 -> 134 IYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKPMFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQLAHMNRHLMPELESVFLMPSKEWSFISSSLVKE
88 -> 91 RGLR

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.5 - Coenzyme A and its modification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016779 - nucleotidyltransferase activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004595 - pantetheine-phosphate adenylyltransferase activity
GO:0042802 - identical protein binding
biological_process GO:0009058 - biosynthetic process
GO:0015937 - coenzyme A biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Miller JR., et al., 2010
[2] Rath CM., et al., 2018
[3] Rothmann M., et al., 2013
External database links:  
DIP:
DIP-35966N
ECOCYC:
PANTEPADENYLYLTRAN-MONOMER
ECOLIWIKI:
b3634
ECOO157CYC:
KDTB-MONOMER
INTERPRO:
IPR014729
INTERPRO:
IPR001980
INTERPRO:
IPR004821
MODBASE:
P0A6I6
PANTHER:
PTHR21342:SF1
PDB:
6B7B
PDB:
6CHQ
PDB:
6CHP
PDB:
1B6T
PDB:
1GN8
PDB:
1H1T
PDB:
1QJC
PDB:
5JBN
PDB:
6B7A
PDB:
6CKW
PDB:
6B7C
PDB:
6B7D
PDB:
6B7E
PDB:
6B7F
PDB:
6CCK
PDB:
6CCL
PDB:
6CCM
PDB:
6CCN
PDB:
6CCO
PDB:
6CCQ
PDB:
6CCS
PDB:
6CHL
PDB:
6CHM
PDB:
6CHN
PDB:
6CHO
PFAM:
PF01467
PRIDE:
P0A6I6
PRINTS:
PR01020
PRODB:
PRO_000022310
REFSEQ:
NP_418091
SMR:
P0A6I6
UNIPROT:
P0A6I6


Operon      
Name: waaA-coaD         
Operon arrangement:
Transcription unit        Promoter
waaA
kdtA-coaD
waaA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4212 3810040 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Miller JR., Thanabal V., Melnick MM., Lall M., Donovan C., Sarver RW., Lee DY., Ohren J., Emerson D., 2010, The use of biochemical and biophysical tools for triage of high-throughput screening hits - A case study with Escherichia coli phosphopantetheine adenylyltransferase., Chem Biol Drug Des 75(5):444-54

 [2] Rath CM., Benton BM., de Vicente J., Drumm JE., Geng M., Li C., Moreau RJ., Shen X., Skepper CK., Steffek M., Takeoka K., Wang L., Wei JR., Xu W., Zhang Q., Feng BY., 2018, Optimization of CoaD Inhibitors against Gram-Negative Organisms through Targeted Metabolomics., ACS Infect Dis 4(3):391-402

 [3] Rothmann M., Kang M., Villa R., Ntai I., La Clair JJ., Kelleher NL., Chapman E., Burkart MD., 2013, Metabolic perturbation of an essential pathway: evaluation of a glycine precursor of coenzyme A., J Am Chem Soc 135(16):5962-5

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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