RegulonDB RegulonDB 10.10: Gene Form
   

epmA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

frdA yjeM epmA frdB DcuR FNR NarL NarL NarL NarL NarL terminator anti-terminator anti-anti-terminator TSS_4998 TSS_4998 epmAp epmAp TSS_4996 (cluster) TSS_4996 (cluster) frdAp frdAp TSS_4995 (cluster) TSS_4995 (cluster) TSS_4994 TSS_4994 TSS_4993 (cluster) TSS_4993 (cluster) TSS_4992 TSS_4992 TSS_4991 (cluster) TSS_4991 (cluster) TSS_4990 (cluster) TSS_4990 (cluster) TSS_4989 TSS_4989 TSS_4988 TSS_4988 TSS_4987 TSS_4987 TSS_4986 TSS_4986 TSS_4985 TSS_4985 TSS_4984 TSS_4984 TSS_4983 TSS_4983 TSS_4982 TSS_4982 TSS_4981 TSS_4981 TSS_4980 (cluster) TSS_4980 (cluster) TSS_4979 TSS_4979 TSS_4978 TSS_4978 TSS_4977 TSS_4977 TSS_4976 TSS_4976 TSS_4975 (cluster) TSS_4975 (cluster) TSS_4974 TSS_4974

Gene      
Name: epmA    Texpresso search in the literature
Synonym(s): ECK4151, EG11211, b4155, genX, poxA, yjeA
Genome position(nucleotides): 4382643 --> 4383620 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.66
External database links:  
ASAP:
 ABE-0013610
ECHOBASE:
 EB1196
ECOLIHUB:
 poxA
OU-MICROARRAY:
 b4155
STRING:
 511145.b4155
COLOMBOS: epmA


Product      
Name: EF-P-lysine lysyltransferase
Synonym(s): EpmA, GenX, PoxA, YjeA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 36.976
Isoelectric point: 4.851
Motif(s):
 
Type Positions Sequence
78 -> 78 E
144 -> 144 A
298 -> 298 A
15 -> 321 NLLKRAAIMAEIRRFFADRGVLEVETPCMSQATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNEEMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEIDPLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPASQASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQHPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFS
103 -> 103 E

 
Classification:
Multifun Terms (GenProtEC)  
  3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.2 - covalent modification, demodification, maturation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016874 - ligase activity
GO:0004812 - aminoacyl-tRNA ligase activity
GO:0004824 - lysine-tRNA ligase activity
GO:0016880 - acid-ammonia (or amide) ligase activity
GO:0000049 - tRNA binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0042803 - protein homodimerization activity
GO:0052868 - protein-lysine lysyltransferase activity
biological_process GO:0006418 - tRNA aminoacylation for protein translation
GO:0006430 - lysyl-tRNA aminoacylation
GO:0043687 - post-translational protein modification
GO:0071468 - cellular response to acidic pH
GO:0071915 - protein-lysine lysylation
GO:0072581 - protein-N6-(L-lysyl)-L-lysine modification to protein-N6-(beta-lysyl)-L-lysine
GO:0018215 - protein phosphopantetheinylation
External database links:  
DIP:
 DIP-10535N
ECOCYC:
 EG11211-MONOMER
ECOLIWIKI:
 b4155
INTERPRO:
 IPR018149
INTERPRO:
 IPR006195
INTERPRO:
 IPR004364
INTERPRO:
 IPR004525
MODBASE:
 P0A8N7
PDB:
 3A5Z
PDB:
 3A5Y
PFAM:
 PF00152
PRIDE:
 P0A8N7
PRINTS:
 PR00982
PROSITE:
 PS50862
REFSEQ:
 NP_418579
SMR:
 P0A8N7
UNIPROT:
 P0A8N7


Operon      
Name: epmA         
Operon arrangement:
Transcription unit        Promoter
epmA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4974 4380562 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4975 (cluster) 4380608 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4976 4380764 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4977 4380768 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4978 4380783 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4979 4380790 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4980 (cluster) 4380795 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4981 4380800 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4982 4380895 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4983 4381317 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4984 4381338 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4985 4381346 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4986 4381467 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4987 4381476 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4988 4381624 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4989 4382039 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4990 (cluster) 4382309 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4991 (cluster) 4382324 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4992 4382327 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4993 (cluster) 4382334 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4994 4382336 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4995 (cluster) 4382398 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4996 (cluster) 4382418 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4998 4383369 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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