RegulonDB RegulonDB 11.1: Gene Form
   

melR gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

adiA melA melR MelR MelR CRP MelR MelR CRP MelR terminator melAp melAp melRp melRp TSS_4922 TSS_4922 adiAp adiAp

Gene      
Name: melR    Texpresso search in the literature
Synonym(s): ECK4111, EG11230, b4118
Genome position(nucleotides): 4340720 <-- 4341628
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.28
External database links:  
ASAP:
 ABE-0013486
CGSC:
 18166
ECHOBASE:
 EB1212
ECOLIHUB:
 melR
OU-MICROARRAY:
 b4118
STRING:
 511145.b4118
COLOMBOS: melR


Product      
Name: DNA-binding transcriptional dual regulator MelR
Synonym(s): MelR
Sequence: Get amino acid sequence Fasta Format
Regulator Family: AraC/XylS
Cellular location: cytosol
Molecular weight: 34.928
Isoelectric point: 8.463
Motif(s):
 
Type Positions Sequence Comment
194 -> 292 SQMLGFIAENYDQALTINDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYSTFGKYVGMSPQQYRKL UniProt: HTH araC/xylS-type.
211 -> 232 NDVAEHVKLNANYAMGIFQRVM UniProt: H-T-H motif.
214 -> 291 AEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYSTFGKYVGMSPQQYRK
222 -> 222 N UniProt: No effect..
259 -> 282 ILDIALTAGFRSSSRFYSTFGKYV UniProt: H-T-H motif.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
  3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0003700 - DNA-binding transcription factor activity
GO:0043565 - sequence-specific DNA binding
biological_process GO:0006355 - regulation of transcription, DNA-templated
Reference(s): [1] Caswell R., et al., 1992
[2] Webster C., et al., 1989
[3] Webster C., et al., 1987
[4] Williams J., et al., 1994
External database links:  
ALPHAFOLD:
 P0ACH8
DIP:
 DIP-10181N
ECOCYC:
 PD00208
ECOLIWIKI:
 b4118
INTERPRO:
 IPR009057
INTERPRO:
 IPR014710
INTERPRO:
 IPR018060
INTERPRO:
 IPR018062
INTERPRO:
 IPR011051
INTERPRO:
 IPR008917
INTERPRO:
 IPR020449
MODBASE:
 P0ACH8
PFAM:
 PF12833
PRIDE:
 P0ACH8
PRINTS:
 PR00032
PRODB:
 PRO_000023199
PROSITE:
 PS01124
PROSITE:
 PS00041
REFSEQ:
 NP_418542
SMART:
 SM00342
UNIPROT:
 P0ACH8


Operon      
Name: melR         
Operon arrangement:
Transcription unit        Promoter
melR


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, MelR
Repressed by: MelR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4922 4340855 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Caswell R., Williams J., Lyddiatt A., Busby S., 1992, Overexpression, purification and characterization of the Escherichia coli MelR transcription activator protein., Biochem J 287 ( Pt 2):493-9

 [2] Webster C., Gardner L., Busby S., 1989, The Escherichia coli melR gene encodes a DNA-binding protein with affinity for specific sequences located in the melibiose-operon regulatory region., Gene 83(2):207-13

 [3] Webster C., Kempsell K., Booth I., Busby S., 1987, Organisation of the regulatory region of the Escherichia coli melibiose operon., Gene 59(2-3):253-63

 [4] Williams J., Michan C., Webster C., Busby S., 1994, Interactions between the Escherichia coli MelR transcription activator protein and operator sequences at the melAB promoter., Biochem J 300 ( Pt 3):757-63

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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