RegulonDB RegulonDB 11.1: Gene Form
   

asmA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

asmA yegH dcd TSS_2401 TSS_2401 TSS_2400 TSS_2400 TSS_2399 TSS_2399

Gene      
Name: asmA    Texpresso search in the literature
Synonym(s): ECK2058, EG11361, b2064, yegA
Genome position(nucleotides): 2139759 <-- 2141612
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.54
External database links:  
ASAP:
 ABE-0006833
ECHOBASE:
 EB1336
ECOLIHUB:
 asmA
OU-MICROARRAY:
 b2064
STRING:
 511145.b2064
COLOMBOS: asmA


Product      
Name: putative assembly protein AsmA
Synonym(s): AsmA, YegA
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space,inner membrane
Molecular weight: 68.963
Isoelectric point: 5.092
Motif(s):
 
Type Positions Sequence Comment
1 -> 22 MRRFLTTLMILLVVLVAGLSAL
4 -> 27 FLTTLMILLVVLVAGLSALVLLVN
23 -> 617 VLLVNPNDFRDYMVKQVAARSGYQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVKQVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQHEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTAAIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTLTEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQGLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLDATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQGQAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVTLNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVYGNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM UniProt: Protein AsmA.
225 -> 511 WQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTLTEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQGLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLDATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQGQAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVTLND
302 -> 321 TANGENGAAQQGQSQSTLPR UniProt: Disordered; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide
  6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
biological_process GO:0090313 - regulation of protein targeting to membrane
Note(s): Note(s): ...[more].
Evidence: [EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Misra R., et al., 1995
[2] Ruiz N., et al., 2021
[3] Xiong X., et al., 1996
External database links:  
ALPHAFOLD:
 P28249
DIP:
 DIP-9175N
ECOCYC:
 EG11361-MONOMER
ECOLIWIKI:
 b2064
INTERPRO:
 IPR007844
PFAM:
 PF05170
PRIDE:
 P28249
PRODB:
 PRO_000022166
REFSEQ:
 NP_416568
UNIPROT:
 P28249


Operon      
Name: asmA         
Operon arrangement:
Transcription unit        Promoter
asmA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2399 2140025 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2400 2140792 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2401 2140838 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Misra R., Miao Y., 1995, Molecular analysis of asmA, a locus identified as the suppressor of OmpF assembly mutants of Escherichia coli K-12., Mol Microbiol 16(4):779-88

 [2] Ruiz N., Davis RM., Kumar S., 2021, YhdP, TamB, and YdbH Are Redundant but Essential for Growth and Lipid Homeostasis of the Gram-Negative Outer Membrane., mBio 12(6):e0271421

 [3] Xiong X., Deeter JN., Misra R., 1996, Assembly-defective OmpC mutants of Escherichia coli K-12., J Bacteriol 178(4):1213-5

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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