RegulonDB RegulonDB 11.1: Gene Form
   

wrbA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

wrbA ycdF yccJ ymdF CsgD CsgD CsgD wrbAp wrbAp

Gene      
Name: wrbA    Texpresso search in the literature
Synonym(s): ECK0995, EG11540, b1004
Genome position(nucleotides): 1067112 <-- 1067708
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.94
External database links:  
ASAP:
 ABE-0003392
CGSC:
 31836
ECHOBASE:
 EB1502
ECOLIHUB:
 wrbA
OU-MICROARRAY:
 b1004
STRING:
 511145.b1004
COLOMBOS: wrbA


Product      
Name: NAD(P)H:quinone oxidoreductase
Synonym(s): WrbA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,membrane
Molecular weight: 20.846
Isoelectric point: 5.677
Motif(s):
 
Type Positions Sequence Comment
2 -> 198 AKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG UniProt: NAD(P)H dehydrogenase (quinone).
4 -> 143 VLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGY
10 -> 15 SMYGHI UniProt: FMN.
78 -> 80 TRF UniProt: FMN.
113 -> 118 STGTGG UniProt: FMN.

 
Classification:
Multifun Terms (GenProtEC)  
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0016020 - membrane
GO:0032991 - protein-containing complex
molecular_function GO:0016491 - oxidoreductase activity
GO:0000166 - nucleotide binding
GO:0050660 - flavin adenine dinucleotide binding
GO:0003955 - NAD(P)H dehydrogenase (quinone) activity
GO:0008753 - NADPH dehydrogenase (quinone) activity
GO:0010181 - FMN binding
GO:0050136 - NADH dehydrogenase (quinone) activity
GO:0042802 - identical protein binding
GO:0050661 - NADP binding
GO:0051287 - NAD binding
biological_process GO:0006979 - response to oxidative stress
Note(s): Note(s): ...[more].
Reference(s): [1] Gotzke H., et al., 2015
[2] Pei Z., et al., 2010
External database links:  
ALPHAFOLD:
 P0A8G6
DIP:
 DIP-36231N
ECOCYC:
 PD01343
ECOLIWIKI:
 b1004
INTERPRO:
 IPR029039
INTERPRO:
 IPR005025
INTERPRO:
 IPR008254
INTERPRO:
 IPR037513
INTERPRO:
 IPR010089
MINT:
 P0A8G6
MODBASE:
 P0A8G6
PDB:
 5F12
PDB:
 4YQE
PDB:
 3ZHO
PDB:
 3B6M
PDB:
 3B6K
PDB:
 3B6J
PDB:
 3B6I
PDB:
 2RG1
PDB:
 2R96
PDB:
 2R97
PFAM:
 PF03358
PRIDE:
 P0A8G6
PRODB:
 PRO_000024221
PROSITE:
 PS50902
REFSEQ:
 NP_415524
SMR:
 P0A8G6
UNIPROT:
 P0A8G6


Operon      
Name: wrbA-yccJ         
Operon arrangement:
Transcription unit        Promoter
wrbA-yccJ


Transcriptional Regulation      
Display Regulation             
Activated by: CsgD


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Gotzke H., Muheim C., Altelaar AF., Heck AJ., Maddalo G., Daley DO., 2015, Identification of Putative Substrates for the Periplasmic Chaperone YfgM in Escherichia coli Using Quantitative Proteomics., Mol Cell Proteomics 14(1):216-26

 [2] Pei Z., Gustavsson T., Roth R., Frejd T., Hagerhall C., 2010, Photolabile ubiquinone analogues for identification and characterization of quinone binding sites in proteins., Bioorg Med Chem 18(10):3457-66

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