RegulonDB RegulonDB 11.2: Gene Form
   

nuoM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nuoL nuoM nuoN nuoK TSS_2539 TSS_2539 TSS_2538 TSS_2538 TSS_2537 TSS_2537 TSS_2536 TSS_2536 TSS_2535 TSS_2535 TSS_2534 TSS_2534 nuoNp1 nuoNp1 TSS_2533 TSS_2533 TSS_2532 TSS_2532 TSS_2531 TSS_2531 TSS_2530 TSS_2530

Gene      
Name: nuoM    Texpresso search in the literature
Synonym(s): ECK2271, EG11773, b2277, nuoA
Genome position(nucleotides): 2391512 <-- 2393041
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.71
External database links:  
ASAP:
 ABE-0007529
CGSC:
 32639
ECHOBASE:
 EB1722
ECOLIHUB:
 nuoM
MIM:
 540000
MIM:
 500001
MIM:
 535000
OU-MICROARRAY:
 b2277
STRING:
 511145.b2277
COLOMBOS: nuoM


Product      
Name: NADH:quinone oxidoreductase subunit M
Synonym(s): NADH:ubiquinone oxidoreductase, membrane subunit M, NuoA, NuoM
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 56.525
Isoelectric point: 8.682
Motif(s):
 
Type Positions Sequence Comment
1 -> 21 MLLPWLILIPFIGGFLCWQTE UniProt: Helical.
30 -> 50 WIALITMGLTLALSLQLWLQG UniProt: Helical.
83 -> 103 IDGLSLLMVVLTGLLGVLAVL UniProt: Helical.
122 -> 142 WILGGVIGVFLAIDMFLFFFF UniProt: Helical.
134 -> 430 IDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLV

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.6 - aerobic respiration
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.4 - energy production/transport --> 1.4.1 - electron donors
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.D - Oxidoreduction-driven Active Transporters
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0030964 - NADH dehydrogenase complex
GO:0045271 - respiratory chain complex I
GO:0045272 - plasma membrane respiratory chain complex I
molecular_function GO:0003954 - NADH dehydrogenase activity
GO:0008137 - NADH dehydrogenase (ubiquinone) activity
GO:0048038 - quinone binding
GO:0048039 - ubiquinone binding
biological_process GO:0009060 - aerobic respiration
GO:0015990 - electron transport coupled proton transport
GO:0042773 - ATP synthesis coupled electron transport
GO:0022904 - respiratory electron transport chain
Note(s): Note(s): ...[more].
Reference(s): [1] Knuuti J., et al., 2013
[2] Zhu S., et al., 2015
External database links:  
ALPHAFOLD:
 P0AFE8
DIP:
 DIP-59255N
ECOCYC:
 NUOM-MONOMER
ECOLIWIKI:
 b2277
INTERPRO:
 IPR001750
INTERPRO:
 IPR010227
INTERPRO:
 IPR003918
PANTHER:
 PTHR43507
PFAM:
 PF00361
PRIDE:
 P0AFE8
PRINTS:
 PR01437
PRODB:
 PRO_000023440
REFSEQ:
 NP_416780
SMR:
 P0AFE8
UNIPROT:
 P0AFE8


Operon      
Name: nuoABCEFGHIJKLMN         
Operon arrangement:
Transcription unit        Promoter
nuoABCEFGHIJKLMN
nuoABCEFGHIJKLMN
nuoMN


Transcriptional Regulation      
Display Regulation             
Activated by: NarL, Fis
Repressed by: FNR, IHF, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2530 2390949 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2531 2391121 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2532 2391223 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2533 2391462 forward nd [RS-EPT-CBR] [3]
  promoter nuoNp1 2391717 reverse nd [COMP-AINF] [4]
  promoter TSS_2534 2393605 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2535 2393635 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2536 2393749 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2537 2394474 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2538 2394595 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2539 2394680 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Knuuti J., Belevich G., Sharma V., Bloch DA., Verkhovskaya M., 2013, A single amino acid residue controls ROS production in the respiratory Complex I from Escherichia coli., Mol Microbiol 90(6):1190-200

 [2] Zhu S., Vik SB., 2015, Constraining the Lateral Helix of Respiratory Complex I by Cross-linking Does Not Impair Enzyme Activity or Proton Translocation., J Biol Chem 290(34):20761-73

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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