RegulonDB RegulonDB 11.2: Gene Form
   

hybF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hybD hybE hybF hybG

Gene      
Name: hybF    Texpresso search in the literature
Synonym(s): ECK2985, EG11804, b2991
Genome position(nucleotides): 3139977 <-- 3140318
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.97
External database links:  
ASAP:
 ABE-0009818
CGSC:
 33427
ECHOBASE:
 EB1752
ECOLIHUB:
 hybF
OU-MICROARRAY:
 b2991
STRING:
 511145.b2991
COLOMBOS: hybF


Product      
Name: hydrogenase maturation protein HybF
Synonym(s): HybF, protein involved with the maturation of hydrogenases 1 and 2
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 12.697
Isoelectric point: 4.889
Motif(s):
 
Type Positions Sequence Comment
1 -> 112 MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAVRFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTGDSLIVKSIEV
2 -> 2 H UniProt: Loss of activity..
3 -> 3 E UniProt: Does not affect activity..
73 -> 73 C UniProt: Affects stability and solubility of the protein, but mutant is still active..
76 -> 76 C UniProt: Affects stability and solubility of the protein, but mutant is still active..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  2 - information transfer --> 2.3 - protein related --> 2.3.3 - posttranslational modification
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0046872 - metal ion binding
GO:0016151 - nickel cation binding
GO:0008270 - zinc ion binding
GO:0016530 - metallochaperone activity
biological_process GO:0006464 - cellular protein modification process
GO:0051604 - protein maturation
Note(s): Note(s): ...[more].
Evidence: [EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Hube M., et al., 2002
External database links:  
ALPHAFOLD:
 P0A703
ECOCYC:
 EG11804-MONOMER
ECOLIWIKI:
 b2991
INTERPRO:
 IPR039002
INTERPRO:
 IPR020538
INTERPRO:
 IPR000688
PANTHER:
 PTHR34535
PFAM:
 PF01155
PRIDE:
 P0A703
PROSITE:
 PS01249
REFSEQ:
 NP_417465
SMR:
 P0A703
UNIPROT:
 P0A703


Operon      
Name: hybOABCDEFG         
Operon arrangement:
Transcription unit        Promoter
hybOABCDEFG


Transcriptional Regulation      
Display Regulation             
Repressed by: NarL, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Hube M., Blokesch M., Bock A., 2002, Network of hydrogenase maturation in Escherichia coli: role of accessory proteins HypA and HybF., J Bacteriol 184(14):3879-85

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