RegulonDB

RegulonDB RegulonDB 11.1: Gene Form

ispF gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	ispF
Synonym(s):	ECK2741, EG11816, b2746, ygbB
Genome position(nucleotides):	2871301 <-- 2871780
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.75
External database links:

ASAP:	ABE-0009013
ECHOBASE:	EB1763
ECOLIHUB:	ispF
OU-MICROARRAY:	b2746
STRING:	511145.b2746
COLOMBOS:	ispF

Product

Name:

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Synonym(s):

IspF, YgbB

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

16.898

Isoelectric point:

6.504

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 154	MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALL
	8 -> 8	D	UniProt: Loss of activity..
	34 -> 35	HS	UniProt: Substrate binding; Sequence Annotation Type: region of interest.
	38 -> 46	DVALHALTD	UniProt: Substrate binding; Sequence Annotation Type: region of interest.
	42 -> 42	H	UniProt: Loss of activity..

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.11 - menaquinone, ubiquinone
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0016829 - lyase activity GO:0046872 - metal ion binding GO:0008685 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity GO:0008270 - zinc ion binding GO:0030145 - manganese ion binding GO:0042802 - identical protein binding
	biological_process	GO:0006744 - ubiquinone biosynthetic process GO:0008299 - isoprenoid biosynthetic process GO:0016114 - terpenoid biosynthetic process GO:0019288 - isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

EG11816-MONOMER

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

ispDF

Operon arrangement:

Transcription unit	Promoter
ispDF

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	ispFp2	2871911		reverse	nd	[COMP-AINF]	[1]
	promoter	ispFp3	2871984		reverse	nd	[COMP-AINF]	[1]

Evidence
[COMP-AINF] Inferred computationally without human oversight

Reference(s)
[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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