RegulonDB

Gene
Name:	napD
Synonym(s):	ECK2199, EG12143, b2207, yojF
Genome position(nucleotides):	2302750 <-- 2303013
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	54.55
External database links:

ASAP:	ABE-0007291
CGSC:	36547
ECHOBASE:	EB2064
ECOLIHUB:	napD
OU-MICROARRAY:	b2207
STRING:	511145.b2207
COLOMBOS:	napD

Product

Name:

NapA signal peptide-binding chaperone NapD

Synonym(s):

NapD, YojF, signal peptide-binding chaperone for NapA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

9.468

Isoelectric point:

3.846

Motif(s):

Type	Positions	Sequence	Comment
	5 -> 81	WQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQLIVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEE

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
		2 - information transfer --> 2.3 - protein related --> 2.3.5 - export, signal peptide cleavage
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0005048 - signal sequence binding
	biological_process	GO:0051224 - negative regulation of protein transport

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IDA-PURIFIED-PROTEIN] Assay of protein purified to homogeneity
[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Dow JM., et al., 2014
[2] Maillard J., et al., 2007
[3] Potter LC., et al., 1999

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

MINT:

PANTHER:

PDB:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

napFDAGHBC-ccmABCDEFGH

Operon arrangement:

Transcription unit	Promoter
napFDAGHBC-ccmABCDEFGH	napFp1
napFDAGHBC-ccmABCDEFGH	napFp2
napFDAGHBC	napFp3
ccmABCDEFGH	ccmAp

Transcriptional Regulation
	Display Regulation
Activated by:	FNR, ModE, NarP, FlhDC
Repressed by:	IscR, NarL, NarP

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Dow JM., Grahl S., Ward R., Evans R., Byron O., Norman DG., Palmer T., Sargent F., 2014, Characterization of a periplasmic nitrate reductase in complex with its biosynthetic chaperone., FEBS J 281(1):246-60
[2] Maillard J., Spronk CA., Buchanan G., Lyall V., Richardson DJ., Palmer T., Vuister GW., Sargent F., 2007, Structural diversity in twin-arginine signal peptide-binding proteins., Proc Natl Acad Sci U S A 104(40):15641-6
[3] Potter LC., Cole JA., 1999, Essential roles for the products of the napABCD genes, but not napFGH, in periplasmic nitrate reduction by Escherichia coli K-12., Biochem J 344 Pt 1:69-76