RegulonDB RegulonDB 11.1: Gene Form
   

nadK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

recN nadK grpE DnaA LexA DnaA LexA CRP terminator anti-terminator anti-anti-terminator TSS_2964 TSS_2964 recNp recNp nadKp2 nadKp2 grpEp grpEp

Gene      
Name: nadK    Texpresso search in the literature
Synonym(s): ECK2611, EG12192, b2615, yfjB, yfjE
Genome position(nucleotides): 2750831 --> 2751709
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.94
Reference(s): [1] Kawai S., et al., 2001
External database links:  
ASAP:
 ABE-0008603
ECHOBASE:
 EB2109
ECOLIHUB:
 nadK
OU-MICROARRAY:
 b2615
STRING:
 511145.b2615
COLOMBOS: nadK


Product      
Name: NAD kinase
Synonym(s): NadK, YfjB, YfjE
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 32.566
Isoelectric point: 6.781
Motif(s):
 
Type Positions Sequence Comment
8 -> 121 IGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIAHELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLTDLDPDNAQQQLADVLEGHY
73 -> 74 DG UniProt: NAD.
147 -> 271 NEVVLHPGKVAHMIEFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSARPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIH
175 -> 175 R UniProt: Does not exhibit NADH kinase activity in addition to NAD kinase activity..
188 -> 193 TAYSLS UniProt: NAD.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.7 - nicotinamide adenine dinucleotide
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0003951 - NAD+ kinase activity
GO:0042802 - identical protein binding
GO:0051287 - NAD binding
biological_process GO:0019674 - NAD metabolic process
GO:0016310 - phosphorylation
GO:0006741 - NADP biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P0A7B3
DIP:
 DIP-48103N
ECOCYC:
 MONOMER0-541
ECOLIWIKI:
 b2615
INTERPRO:
 IPR017438
INTERPRO:
 IPR017437
INTERPRO:
 IPR016064
INTERPRO:
 IPR002504
PFAM:
 PF01513
PFAM:
 PF20143
PRIDE:
 P0A7B3
REFSEQ:
 NP_417105
SMR:
 P0A7B3
SWISSMODEL:
 P0A7B3
UNIPROT:
 P0A7B3


Operon      
Name: nadK         
Operon arrangement:
Transcription unit        Promoter
nadK


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter nadKp2 2750763 forward nd [COMP-AINF] [2]
  promoter TSS_2964 2752658 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Kawai S., Mori S., Mukai T., Hashimoto W., Murata K., 2001, Molecular characterization of Escherichia coli NAD kinase., Eur J Biochem 268(15):4359-65

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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