RegulonDB RegulonDB 10.9: Gene Form
   

glcB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

glcB glcA glcG C0719 anti-anti-terminator anti-terminator terminator TSS_3299 TSS_3299 glcAp10 glcAp10 glcAp12 glcAp12

Gene      
Name: glcB    Texpresso search in the literature
Synonym(s): ECK2970, EG20080, b2976, glc
Genome position(nucleotides): 3121634 <-- 3123805 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.45
External database links:  
ASAP:
ABE-0009767
CGSC:
711
ECHOBASE:
EB4141
ECOLIHUB:
glcB
OU-MICROARRAY:
b2976
STRING:
511145.b2976
COLOMBOS: glcB


Shine dalgarno      
Sequence: taagcgaaaaCGAGGAgataaacAAT


Product      
Name: malate synthase G
Synonym(s): Glc, GlcB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 80.488
Isoelectric point: 6.084
Motif(s):
 
Type Positions Sequence
18 -> 697 RFVDEEVLPGTGLDAAAFWRNFDEIVHDLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVETTGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDPQRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVGYRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVAAVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSLLFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPKMHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFINTGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGMWAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFNAEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIHNVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAGNFANSCAFKAASDLI
125 -> 126 RY
338 -> 338 R
617 -> 617 C
2 -> 723 SQTITQSRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVHDLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVETTGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDPQRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVGYRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVAAVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSLLFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPKMHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFINTGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGMWAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFNAEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIHNVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAGNFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.21 - glyoxylate degradation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0004474 - malate synthase activity
GO:0000287 - magnesium ion binding
biological_process GO:0006097 - glyoxylate cycle
GO:0006099 - tricarboxylic acid cycle
GO:0009436 - glyoxylate catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Anstrom DM., et al., 2005
[2] Choi SY., et al., 2016
[3] Dahiya V., et al., 2013
[4] Hu W., et al., 2012
[5] Kumar R., et al., 2011
[6] Miyanoiri Y., et al., 2013
[7] Rui B., et al., 2010
[8] Sheppard D., et al., 2009
[9] Tugarinov V., et al., 2004
[10] Tugarinov V., et al., 2006
External database links:  
DIP:
DIP-9761N
DISPROT:
DP01837
ECOCYC:
MALSYNG-MONOMER
ECOLIWIKI:
b2976
INTERPRO:
IPR023310
INTERPRO:
IPR011076
INTERPRO:
IPR006253
INTERPRO:
IPR001465
MINT:
P37330
MODBASE:
P37330
PANTHER:
PTHR42739
PDB:
1D8C
PDB:
1P7T
PDB:
2JQX
PDB:
1Y8B
PFAM:
PF01274
PRIDE:
P37330
PRODB:
PRO_000022772
REFSEQ:
NP_417450
SMR:
P37330
UNIPROT:
P37330


Operon      
Name: glcDEFGBA         
Operon arrangement:
Transcription unit        Promoter
glcDEFGBA
glcBA


Transcriptional Regulation      
Display Regulation             
Activated by: GlcC, IHF
Repressed by: ArcA, PdhR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter glcAp12 3121321 reverse Similarity to the consensus
Read more >
[ICWHO] [11]
  promoter glcAp10 3121364 reverse Similarity to the consensus
Read more >
[ICWHO] [11]
  promoter TSS_3299 3121581 reverse nd [RS-EPT-CBR] [12]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Anstrom DM., Colip L., Moshofsky B., Hatcher E., Remington SJ., 2005, Systematic replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G: effect on crystallization., Acta Crystallogr Sect F Struct Biol Cryst Commun 61(Pt 12):1069-74

 [2] Choi SY., Park SJ., Kim WJ., Yang JE., Lee H., Shin J., Lee SY., 2016, One-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli., Nat Biotechnol 34(4):435-40

 [3] Dahiya V., Chaudhuri TK., 2013, Functional intermediate in the refolding pathway of a large and multidomain protein malate synthase G., Biochemistry 52(26):4517-30

 [4] Hu W., Namanja AT., Wong S., Chen Y., 2012, Selective editing of Val and Leu methyl groups in high molecular weight protein NMR., J Biomol NMR 53(2):113-24

 [5] Kumar R., Bhakuni V., 2011, Comparative analysis of malate synthase G from Mycobacterium tuberculosis and E. coli: role of ionic interaction in modulation of structural and functional properties., Int J Biol Macromol 49(5):917-22

 [6] Miyanoiri Y., Takeda M., Okuma K., Ono AM., Terauchi T., Kainosho M., 2013, Differential isotope-labeling for Leu and Val residues in a protein by E. coli cellular expression using stereo-specifically methyl labeled amino acids., J Biomol NMR 57(3):237-49

 [7] Rui B., Shen T., Zhou H., Liu J., Chen J., Pan X., Liu H., Wu J., Zheng H., Shi Y., 2010, A systematic investigation of Escherichia coli central carbon metabolism in response to superoxide stress., BMC Syst Biol 4:122

 [8] Sheppard D., Guo C., Tugarinov V., 2009, Methyl-detected 'out-and-back' NMR experiments for simultaneous assignments of Alabeta and Ilegamma2 methyl groups in large proteins., J Biomol NMR 43(4):229-38

 [9] Tugarinov V., Hwang PM., Kay LE., 2004, Nuclear magnetic resonance spectroscopy of high-molecular-weight proteins., Annu Rev Biochem 73:107-46

 [10] Tugarinov V., Kanelis V., Kay LE., 2006, Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy., Nat Protoc 1(2):749-54

 [11] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [12] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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