RegulonDB RegulonDB 10.8: Gene Form
   

xylF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

xylG xylA xylF xylH Fis XylR XylR Fis Fis CRP XylR AraC XylR AraC anti-terminator xylFp xylFp xylAp xylAp

Gene      
Name: xylF    Texpresso search in the literature
Synonym(s): ECK3555, EG20252, b3566, xylT
Genome position(nucleotides): 3731131 --> 3732123 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
44.01
External database links:  
ASAP:
ABE-0011646
CGSC:
17773
ECHOBASE:
EB4153
OU-MICROARRAY:
b3566
PortEco:
xylF
STRING:
511145.b3566
COLOMBOS: xylF


Product      
Name: xylose ABC transporter periplasmic binding protein
Synonym(s): XBP, XylF, XylT
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 35.734
Isoelectric point: 5.258
Motif(s):
 
Type Positions Sequence
284 -> 307 GQEPKADTTLNNGLKDVPSRLLTP
1 -> 23 MKIKNILLTLCTSLLLTNVAAHA
242 -> 244 SGQ
38 -> 286 ERWQKDRDIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQE
283 -> 284 NG

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.p - ABC superfamily, periplasmic binding component
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
molecular_function GO:0030246 - carbohydrate binding
GO:0048029 - monosaccharide binding
biological_process GO:0008643 - carbohydrate transport
GO:0042732 - D-xylose metabolic process
GO:0015752 - D-ribose transmembrane transport
GO:0015753 - D-xylose transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Nicholes N., et al., 2015
[2] Ribeiro LF., et al., 2013
[3] Ribeiro LF., et al., 2016
External database links:  
ECOCYC:
XYLF-MONOMER
ECOLIWIKI:
b3566
INTERPRO:
IPR013456
INTERPRO:
IPR028082
INTERPRO:
IPR025997
MODBASE:
P37387
PDB:
3M9X
PDB:
3M9W
PDB:
3MA0
PFAM:
PF13407
PRIDE:
P37387
PRODB:
PRO_000024242
REFSEQ:
NP_418023
SMR:
P37387
UNIPROT:
P37387


Operon      
Name: xylFGHR         
Operon arrangement:
Transcription unit        Promoter
xylFGHR
xylR


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, XylR
Repressed by: Fis


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Nicholes N., Tullman J., Ribeiro LF., Fuzo CA., Vieira DS., Furtado GP., Ostermeier M., Ward RJ., 2015, Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase., Biotechnol Biofuels 8:118

 [2] Ribeiro LF., Bressan F., Furtado GP., Meireles F., Ward RJ., 2013, D-xylose detection in Escherichia coli by a xylose binding protein-dependent response., J Biotechnol 168(4):440-5

 [3] Ribeiro LF., Tullman J., Nicholes N., Silva SR., Vieira DS., Ostermeier M., Ward RJ., 2016, A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination., Biotechnol Biofuels 9:119


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