RegulonDB RegulonDB 11.1: Gene Form
   

flgI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

flgK flgI flgJ flgH TSS_1348 TSS_1348 flgKp flgKp TSS_1347 TSS_1347 TSS_1346 TSS_1346

Gene      
Name: flgI    Texpresso search in the literature
Synonym(s): ECK1065, G365, b1080, flaM
Genome position(nucleotides): 1136274 --> 1137371
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.19
External database links:  
ASAP:
 ABE-0003656
CGSC:
 764
ECHOBASE:
 EB4162
ECOLIHUB:
 flgI
OU-MICROARRAY:
 b1080
STRING:
 511145.b1080
COLOMBOS: flgI


Product      
Name: flagellar P-ring protein
Synonym(s): FlaM, FlgI
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space,cell projection
Molecular weight: 38.169
Isoelectric point: 9.648
Motif(s):
 
Type Positions Sequence Comment
1 -> 19 MIKFLSALILLLVTTAAQA
20 -> 365 ERIRDLTSVQGVRQNSLIGYGLVVGLDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQTIDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQLNGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDARTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVAQGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRALNALGATPMDLMSILQSMQSAGCLRAKLEII UniProt: Flagellar P-ring protein.
21 -> 364 RIRDLTSVQGVRQNSLIGYGLVVGLDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQTIDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQLNGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDARTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVAQGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRALNALGATPMDLMSILQSMQSAGCLRAKLEI
22 -> 22 I I → C: cells carrying the mutation are weakly motile
40 -> 40 G G → C: cells carrying the mutation are non-motile and lack flagella

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.12 - flagellum
  5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
  6 - cell structure --> 6.4 - flagella
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
GO:0009288 - bacterial-type flagellum
GO:0009425 - bacterial-type flagellum basal body
GO:0009428 - bacterial-type flagellum basal body, distal rod, P ring
molecular_function GO:0005198 - structural molecule activity
biological_process GO:0006974 - cellular response to DNA damage stimulus
GO:0071973 - bacterial-type flagellum-dependent cell motility
GO:0097588 - archaeal or bacterial-type flagellum-dependent cell motility
Note(s): Note(s): ...[more].
Evidence: [EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Hizukuri Y., et al., 2010
[2] Hizukuri Y., et al., 2008
[3] Jones CJ., et al., 1987
External database links:  
ALPHAFOLD:
 P0A6S3
DIP:
 DIP-47979N
ECOCYC:
 FLGI-FLAGELLAR-P-RING
ECOLIWIKI:
 b1080
INTERPRO:
 IPR001782
PANTHER:
 PTHR30381
PFAM:
 PF02119
PRIDE:
 P0A6S3
PRINTS:
 PR01010
PRODB:
 PRO_000022644
REFSEQ:
 NP_415598
UNIPROT:
 P0A6S3


Operon      
Name: flgBCDEFGHIJ         
Operon arrangement:
Transcription unit        Promoter
flgBCDEFGHIJ


Transcriptional Regulation      
Display Regulation             
Activated by: FlhDC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1346 1137124 forward nd [RS-EPT-CBR] [4]
  promoter TSS_1347 1137587 forward nd [RS-EPT-CBR] [4]
  promoter TSS_1348 1138353 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Hizukuri Y., Kojima S., Homma M., 2010, Disulphide cross-linking between the stator and the bearing components in the bacterial flagellar motor., J Biochem 148(3):309-18

 [2] Hizukuri Y., Kojima S., Yakushi T., Kawagishi I., Homma M., 2008, Systematic Cys mutagenesis of FlgI, the flagellar P-ring component of Escherichia coli., Microbiology 154(Pt 3):810-7

 [3] Jones CJ., Homma M., Macnab RM., 1987, Identification of proteins of the outer (L and P) rings of the flagellar basal body of Escherichia coli., J Bacteriol 169(4):1489-92

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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