RegulonDB RegulonDB 11.1: Gene Form
   

flgJ gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

flgK flgI flgJ flgH TSS_1348 TSS_1348 flgKp flgKp TSS_1347 TSS_1347 TSS_1346 TSS_1346

Gene      
Name: flgJ    Texpresso search in the literature
Synonym(s): ECK1066, G366, b1081, flaZ
Genome position(nucleotides): 1137371 --> 1138312
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.67
External database links:  
ASAP:
 ABE-0003658
CGSC:
 751
ECHOBASE:
 EB4020
ECOLIHUB:
 flgJ
OU-MICROARRAY:
 b1081
STRING:
 511145.b1081
COLOMBOS: flgJ


Product      
Name: putative peptidoglycan hydrolase FlgJ
Synonym(s): FlaZ, FlgJ
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 34.475
Isoelectric point: 8.609
Motif(s):
 
Type Positions Sequence Comment
50 -> 97 KSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQ
148 -> 313 DSKAFLAQLSLPAQLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF UniProt: Catalytic; Sequence Annotation Type: region of interest.
161 -> 295 QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITTTEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAGYATDPHYARKLTNMIQQMK

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.12 - flagellum
  5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
  6 - cell structure --> 6.4 - flagella
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
molecular_function GO:0016787 - hydrolase activity
GO:0016798 - hydrolase activity, acting on glycosyl bonds
GO:0004040 - amidase activity
biological_process GO:0008152 - metabolic process
GO:0071555 - cell wall organization
GO:0071973 - bacterial-type flagellum-dependent cell motility
GO:0044780 - bacterial-type flagellum assembly
GO:0044781 - bacterial-type flagellum organization
Note(s): Note(s): ...[more].
Evidence: [EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Juhas M., et al., 2015
External database links:  
ALPHAFOLD:
 P75942
CAZY:
 GH73
ECOCYC:
 G366-MONOMER
ECOLIWIKI:
 b1081
INTERPRO:
 IPR019301
INTERPRO:
 IPR013377
INTERPRO:
 IPR002901
PFAM:
 PF01832
PFAM:
 PF10135
PRIDE:
 P75942
PRODB:
 PRO_000022645
REFSEQ:
 NP_415599
SMART:
 SM00047
SMR:
 P75942
UNIPROT:
 P75942


Operon      
Name: flgBCDEFGHIJ         
Operon arrangement:
Transcription unit        Promoter
flgBCDEFGHIJ


Transcriptional Regulation      
Display Regulation             
Activated by: FlhDC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1346 1137124 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1347 1137587 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1348 1138353 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Juhas M., Ajioka JW., 2015, Identification and Validation of Novel Chromosomal Integration and Expression Loci in Escherichia coli Flagellar Region 1., PLoS One 10(3):e0123007

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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