RegulonDB

Gene
Name:	flgM
Synonym(s):	ECK1056, G369, b1071
Genome position(nucleotides):	1129835 <-- 1130128
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.72
External database links:

ASAP:	ABE-0003634
ECHOBASE:	EB2530
ECOLIHUB:	flgM
OU-MICROARRAY:	b1071
STRING:	511145.b1071
COLOMBOS:	flgM

Product

Name:

anti-sigma factor for FliA (σ²⁸)

Synonym(s):

FlgM

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

10.341

Isoelectric point:

5.054

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 39	MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTST	UniProt: Disordered; Sequence Annotation Type: region of interest.
	40 -> 88	SVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALIN

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.1 - sigma factors, anti-sigmafactors
		5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
		6 - cell structure --> 6.4 - flagella
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0016989 - sigma factor antagonist activity
	biological_process	GO:0045892 - negative regulation of transcription, DNA-templated GO:0044781 - bacterial-type flagellum organization GO:0045861 - negative regulation of proteolysis

Note(s):

Note(s): ...[more].

Evidence:

[EXP] Inferred from experiment

Reference(s):

[1] Barembruch C., et al., 2007
[2] Dudin O., et al., 2013
[3] Heel T., et al., 2013

External database links:

ALPHAFOLD:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

flgAMN

Operon arrangement:

Transcription unit	Promoter
flgMN	flgMp
flgAMN	flgAp

Transcriptional Regulation
	Display Regulation
Activated by:	FlhDC
Repressed by:	CsgD

Regulation by small RNA
	Display Regulation
small RNA	omrB, omrA

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	flgNp1	1129914	reverse	nd	[COMP-AINF]	[4]
promoter	TSS_1342	1130164	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_1343	1130193	reverse	nd	[RS-EPT-CBR]	[5]

Evidence
[COMP-AINF] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Barembruch C., Hengge R., 2007, Cellular levels and activity of the flagellar sigma factor FliA of Escherichia coli are controlled by FlgM-modulated proteolysis., Mol Microbiol 65(1):76-89
[2] Dudin O., Lacour S., Geiselmann J., 2013, Expression dynamics of RpoS/Crl-dependent genes in Escherichia coli., Res Microbiol 164(8):838-47
[3] Heel T., Vogel GF., Lammirato A., Schneider R., Auer B., 2013, FlgM as a secretion moiety for the development of an inducible type III secretion system., PLoS One 8(3):e59034
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.