RegulonDB RegulonDB 10.8: Gene Form
   

rihA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hscC rihA gltL rihAp3 rihAp3 TSS_777 (cluster) TSS_777 (cluster) hscCp7 hscCp7 hscCp5 hscCp5

Gene      
Name: rihA    Texpresso search in the literature
Synonym(s): ECK0644, G6358, b0651, ybeK
Genome position(nucleotides): 683477 <-- 684412 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.85
External database links:  
ASAP:
 ABE-0002227
ECHOBASE:
 EB2563
OU-MICROARRAY:
 b0651
PortEco:
 rihA
STRING:
 511145.b0651
COLOMBOS: rihA


Product      
Name: pyrimidine-specific ribonucleoside hydrolase RihA
Synonym(s): RihA, YbeK, ribonucleoside hydrolase 1 (pyrimidine-specific)
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 33.823
Isoelectric point: 4.596
Motif(s):
 
Type Positions Sequence
4 -> 300 PILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEKTLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNCTAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWTPAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAELLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTSVERWVGVETQGKYTQGMTVVDYYYLTGNKPNATVMVDVDRQGFV

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0032991 - protein-containing complex
molecular_function GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0016798 - hydrolase activity, acting on glycosyl bonds
GO:0008477 - purine nucleosidase activity
GO:0016799 - hydrolase activity, hydrolyzing N-glycosyl compounds
GO:0045437 - uridine nucleosidase activity
GO:0005509 - calcium ion binding
GO:0042802 - identical protein binding
GO:0050263 - ribosylpyrimidine nucleosidase activity
biological_process GO:0008152 - metabolic process
GO:0006206 - pyrimidine nucleobase metabolic process
GO:0015949 - nucleobase-containing small molecule interconversion
GO:0006152 - purine nucleoside catabolic process
GO:0046133 - pyrimidine ribonucleoside catabolic process
GO:0051289 - protein homotetramerization
External database links:  
ECOCYC:
 G6358-MONOMER
ECOLIWIKI:
 b0651
INTERPRO:
 IPR023186
INTERPRO:
 IPR022975
INTERPRO:
 IPR036452
INTERPRO:
 IPR015910
INTERPRO:
 IPR001910
MODBASE:
 P41409
PANTHER:
 PTHR12304
PDB:
 3G5I
PDB:
 1YOE
PFAM:
 PF01156
PRIDE:
 P41409
PRODB:
 PRO_000023756
PROSITE:
 PS01247
REFSEQ:
 NP_415184
SMR:
 P41409
SWISSMODEL:
 P41409
UNIPROT:
 P41409


Operon      
Name: rihA         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter hscCp5 683482 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter hscCp7 683594 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter TSS_777 (cluster) 684440 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter rihAp3 684602 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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