RegulonDB RegulonDB 11.1: Gene Form
   

mgsA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

helD yccT mgsA anti-anti-terminator anti-terminator terminator mgsAp mgsAp TSS_1288 TSS_1288 TSS_1287 TSS_1287

Gene      
Name: mgsA    Texpresso search in the literature
Synonym(s): ECK0954, G6497, b0963, mgs, yccG
Genome position(nucleotides): 1026557 <-- 1027015
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.72
External database links:  
ASAP:
 ABE-0003259
ECHOBASE:
 EB2213
ECOLIHUB:
 mgsA
OU-MICROARRAY:
 b0963
STRING:
 511145.b0963
COLOMBOS: mgsA


Product      
Name: methylglyoxal synthase
Synonym(s): Mgs, MgsA, YccG
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 16.919
Isoelectric point: 6.634
Motif(s):
 
Type Positions Sequence Comment
1 -> 2 ME UniProt: In Ref. 1; J04726..
6 -> 152 RTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYATGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDVKALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK UniProt: MGS-like.
20 -> 20 D UniProt: Strongly decreased catalytic efficiency..
26 -> 118 LMSWVERHQPLLEQHVLYATGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDVKALLRLATVWNIPVAT
45 -> 48 TGTT UniProt: Substrate binding; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.23 - methylglyoxal metabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016829 - lyase activity
GO:0008929 - methylglyoxal synthase activity
GO:0042802 - identical protein binding
biological_process GO:0019242 - methylglyoxal biosynthetic process
GO:0034214 - protein hexamerization
External database links:  
ALPHAFOLD:
 P0A731
DIP:
 DIP-48252N
ECOCYC:
 METHGLYSYN-MONOMER
ECOLIWIKI:
 b0963
INTERPRO:
 IPR036914
INTERPRO:
 IPR004363
INTERPRO:
 IPR018148
INTERPRO:
 IPR011607
MODBASE:
 P0A731
PANTHER:
 PTHR30492
PDB:
 1S8A
PDB:
 1S89
PDB:
 1IK4
PDB:
 1B93
PDB:
 1EGH
PFAM:
 PF02142
PRIDE:
 P0A731
PRODB:
 PRO_000023226
PROSITE:
 PS01335
PROSITE:
 PS51855
REFSEQ:
 NP_415483
SMART:
 SM00851
SMR:
 P0A731
UNIPROT:
 P0A731


Operon      
Name: mgsA         
Operon arrangement:
Transcription unit        Promoter
mgsA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1287 1025511 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1288 1027030 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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