RegulonDB RegulonDB 11.1: Gene Form
   

efeU gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

putP efeO efeU CpxR efeOp1 efeOp1 efeOp2 efeOp2 efeUp efeUp

Gene      
Name: efeU    Texpresso search in the literature
Synonym(s): ECK1007, G6525, b1016, b1017, b4490, ycdN
Type: Pseudo Gene
Genome position(nucleotides): 1081356 --> 1082185
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.22
Note(s): efeU_1 and efeU_2 are two fragments of a pseudogene (efeU*). A functional efeU (ycdN) gene in E. coli Nissle 1917 encodes a ferrous iron permease Grosse C, Scherer J, Koch D, Otto M, Taudte N, Grass G,2006; a functional efeUOB operon in enterohaemorrhagic E. coli O157:H7 encodes a tripartite, acid-induced Fe(II) transport system Cao J, Woodhall MR, Alvarez J, Cartron ML, Andrews SC,2007.
Analysis of transcriptional efe-lacZ fusions suggest that efeU*OB forms an operon in E. coli K-12 which is transcribed from a promoter located upstream of efeU* Cao J, Woodhall MR, Alvarez J, Cartron ML, Andrews SC,2007. efeU* in E. coli K-12 is disrupted by a frameshift mutation leaving the efeU_1 and efeU_2 fragments nonfunctional; EfeO and EfeB are produced despite the frameshift mutation Grosse C, Scherer J, Koch D, Otto M, Taudte N, Grass G,2006
efeU*OB transcription in E. coli K-12 is subject to Fe(II)-Fur control in response to iron McHugh JP,2003. Grosse C, Scherer J, Koch D, Otto M, Taudte N, Grass G,2006. Cao J, Woodhall MR, Alvarez J, Cartron ML, Andrews SC,2007. The efeU*OB operon is induced at acid pH and subject to CpxAR dependent repression at alkaline pH Cao J, Woodhall MR, Alvarez J, Cartron ML, Andrews SC,2007.
Deletion of efeU*, efeO, or efeB does not result in any growth defect under iron limitation Cao J, Woodhall MR, Alvarez J, Cartron ML, Andrews SC,2007 Low copy-number expression of a 'corrected' efeUOB operon in an E. coli K-12 strain lacking lacking all known iron-transport pathways confers a growth advantage under iron restricted conditions Cao J, Woodhall MR, Alvarez J, Cartron ML, Andrews SC,2007.
Expression of EfeU decreases in early osmotic upshift (0.4 M NaCl) 12218039 efeU_2 expression decreases under anaerobic conditions in both a wild-type and a Δfur mutant strain Kang Y,2005
efe: elemental ferrous iron uptake Grosse C, Scherer J, Koch D, Otto M, Taudte N, Grass G,2006.
Reference(s): [1] Masse E., et al., 2005
[2] Ren D., et al., 2004
External database links:  
ASAP:
 ABE-0003441
ASAP:
 ABE-0285091
ASAP:
 ABE-0003443
ECHOBASE:
 EB3620
ECOLIHUB:
 efeU_2
ECOLIHUB:
 efeU_1
INTERPRO:
 IPR004923
INTERPRO:
 IPR005217
OU-MICROARRAY:
 b1017
OU-MICROARRAY:
 b1016
UNIPROT:
 P75901
COLOMBOS: efeU


Product      
Name: inactive ferrous iron permease EfeU
Sequence: Get amino acid sequence Fasta Format
Isoelectric point: 11.711
Motif(s):
 
Type Positions Sequence Comment
2 -> 22 FVPFLIMLREGLEAALIVSLI UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
Gene Ontology Terms (GO)  
cellular_component GO:0033573 - high-affinity iron permease complex
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005381 - iron ion transmembrane transporter activity
GO:0015093 - ferrous iron transmembrane transporter activity
biological_process GO:0034755 - iron ion transmembrane transport
External database links:  
ECOCYC:
 MONOMER0-4386
UNIPROT:
 P75901


Operon      
Name: efeU         
Operon arrangement:
Transcription unit        Promoter
efeU


Transcriptional Regulation      
Display Regulation             
Repressed by: CpxR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter efeOp2 1082154 forward nd [COMP-AINF] [3]
  promoter efeOp1 1082243 forward nd [COMP-AINF] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Masse E., Vanderpool CK., Gottesman S., 2005, Effect of RyhB small RNA on global iron use in Escherichia coli., J Bacteriol 187(20):6962-71

 [2] Ren D., Bedzyk LA., Ye RW., Thomas SM., Wood TK., 2004, Stationary-phase quorum-sensing signals affect autoinducer-2 and gene expression in Escherichia coli., Appl Environ Microbiol 70(4):2038-43

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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