RegulonDB

Gene
Name:	bluF
Synonym(s):	ECK1150, G6603, b1163, ycgF
Genome position(nucleotides):	1214264 <-- 1215475 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	43.56
External database links:

ASAP:	ABE-0003903
ECHOBASE:	EB3646
ECOLIHUB:	ycgF
OU-MICROARRAY:	b1163
STRING:	511145.b1163
COLOMBOS:	bluF

Product

Name:

blue light- and temperature-regulated antirepressor BluF

Synonym(s):

Blrp, BluF, YcgF, blue light-responsive regulator of BluR

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

45.295

Isoelectric point:

4.813

Motif(s):

Type	Positions	Sequence
	193 -> 195	IVQ
	2 -> 92	LTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNGSHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGM
	155 -> 403	DGSDKELDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLNGIPSIAWPEKK
	98 -> 144	RLHERDDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEI
	173 -> 388	HFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTADLKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAK

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional
Gene Ontology Terms (GO)
	cellular_component	GO:0005886 - plasma membrane
	molecular_function	GO:0140297 - DNA-binding transcription factor binding GO:0050660 - flavin adenine dinucleotide binding GO:0009881 - photoreceptor activity GO:0071949 - FAD binding GO:0009882 - blue light photoreceptor activity
	biological_process	GO:0050896 - response to stimulus GO:0043433 - negative regulation of DNA-binding transcription factor activity GO:0018298 - protein-chromophore linkage GO:0009637 - response to blue light GO:0009785 - blue light signaling pathway

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[IDA] Inferred from direct assay

Reference(s):

[1] Rajagopal S., et al., 2004
[2] Terazima M. 2011
[3] Tschowri N., et al., 2009
[4] Weber S., et al., 2011
[5] Yee EF., et al., 2019

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

PFAM:

PFAM:

PRIDE:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMART:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	RcdA

Reference(s)
[1] Rajagopal S., Key JM., Purcell EB., Boerema DJ., Moffat K., 2004, Purification and initial characterization of a putative blue light-regulated phosphodiesterase from Escherichia coli., Photochem Photobiol 80(3):542-7
[2] Terazima M., 2011, Time-dependent intermolecular interaction during protein reactions., Phys Chem Chem Phys 13(38):16928-40
[3] Tschowri N., Busse S., Hengge R., 2009, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli., Genes Dev 23(4):522-34
[4] Weber S., Schroeder C., Kacprzak S., Mathes T., Kowalczyk RM., Essen LO., Hegemann P., Schleicher E., Bittl R., 2011, Light-generated paramagnetic intermediates in BLUF domains., Photochem Photobiol 87(3):574-83
[5] Yee EF., Chandrasekaran S., Lin C., Crane BR., 2019, Physical methods for studying flavoprotein photoreceptors., Methods Enzymol 620:509-544
[6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78