RegulonDB

Gene
Name:	ortT
Synonym(s):	ECK1439, G6756, b1445, ydcX
Genome position(nucleotides):	1517389 --> 1517562
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	44.83
External database links:

ASAP:	ABE-0004818
ECHOBASE:	EB3530
ECOLIHUB:	ydcX
OU-MICROARRAY:	b1445
STRING:	511145.b1445
COLOMBOS:	ortT

Product

Name:

orphan toxin OrtT

Synonym(s):

OrtT, YdcX

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

6.646

Isoelectric point:

10.452

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 1	M	UniProt: Not toxic as no protein is produced..
	4 -> 57	YQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATWPMSFPVALLFSLF
	6 -> 26	HMLVFYAVMAAIAFLITWFLS	UniProt: Helical.
	34 -> 54	FLSAFLVGATWPMSFPVALLF	UniProt: Helical.
	38 -> 38	F	alternate sequence F → R reduces OrtT toxicity

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		5 - cell processes --> 5.5 - adaptations --> 5.5.6 - other (mechanical, nutritional, oxidative stress)
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	biological_process	GO:0001896 - autolysis

Note(s):

Note(s): ...[more].

External database links:

ALPHAFOLD:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

ortT

Operon arrangement:

Transcription unit	Promoter
ortT

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_1816	1517209	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_1817 (cluster)	1517218	reverse	nd	[RS-EPT-CBR]	[1]
promoter	ydcXp1	1517221	forward	nd	[COMP-AINF]	[2]
promoter	ydcXp7	1517236	forward	nd	[COMP-AINF]	[2]
promoter	yncLp4	1517246	reverse	nd	[COMP-AINF]	[2]
promoter	ydcXp9	1517354	forward	nd	[COMP-AINF]	[2]
promoter	ydcYp3	1517610	forward	nd	[COMP-AINF]	[2]
promoter	TSS_1818 (cluster)	1517612	forward	nd	[RS-EPT-CBR]	[1]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[COMP-AINF] Inferred computationally without human oversight

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78