RegulonDB

Gene
Name:	safA
Synonym(s):	ECK4475, G6790, b1500, yneN
Genome position(nucleotides):	1583762 <-- 1583959
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	37.37
External database links:

ASAP:	ABE-0004994
ECOLIHUB:	yneN
OU-MICROARRAY:	b1500
STRING:	511145.b1500
COLOMBOS:	safA

Product

Name:

two-component system connector SafA

Synonym(s):

SafA, YneN

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

7.272

Isoelectric point:

9.224

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 64	MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLSISEMGRIARDLTHFIINSLQ
	19 -> 40	IMSAIVVVLLLTLTLIAIFSAI

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.3 - protein related
		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.3 - inhibition / activation of enzymes
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0005515 - protein binding GO:0030295 - protein kinase activator activity
	biological_process	GO:0010447 - response to acidic pH

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IDA] Inferred from direct assay
[EXP-IPI] Inferred from physical interaction

Reference(s):

[1] Eguchi Y., et al., 2011
[2] Eguchi Y., et al., 2007
[3] Herzberg M., et al., 2006
[4] Svanberg Frisinger F., et al., 2021

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

safA-ydeO

Operon arrangement:

Transcription unit	Promoter
safA-ydeO	safAp
ydeO	ydeOp

Transcriptional Regulation
	Display Regulation
Activated by:	EvgA, RcsB
Repressed by:	PhoP, YdeO

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Eguchi Y., Ishii E., Hata K., Utsumi R., 2011, Regulation of acid resistance by connectors of two-component signal transduction systems in Escherichia coli., J Bacteriol 193(5):1222-8
[2] Eguchi Y., Itou J., Yamane M., Demizu R., Yamato F., Okada A., Mori H., Kato A., Utsumi R., 2007, B1500, a small membrane protein, connects the two-component systems EvgS/EvgA and PhoQ/PhoP in Escherichia coli., Proc Natl Acad Sci U S A 104(47):18712-7
[3] Herzberg M., Kaye IK., Peti W., Wood TK., 2006, YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport., J Bacteriol 188(2):587-98
[4] Svanberg Frisinger F., Jana B., Donadio S., Guardabassi L., 2021, In Silico Prediction and Prioritization of Novel Selective Antimicrobial Drug Targets in Escherichia coli., Antibiotics (Basel) 10(6)