RegulonDB RegulonDB 10.8: Gene Form
   

rstA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rstB rstA ydgC folM PhoP tusp2 tusp2 rstAp rstAp TSS_1894 TSS_1894 ydgCp3 ydgCp3

Gene      
Name: rstA    Texpresso search in the literature
Synonym(s): ECK1603, G6864, b1608, urpT
Genome position(nucleotides): 1682159 --> 1682878 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.31
External database links:  
ASAP:
 ABE-0005370
ECHOBASE:
 EB2982
OU-MICROARRAY:
 b1608
PortEco:
 rstA
STRING:
 511145.b1608
COLOMBOS: rstA


Product      
Name: DNA-binding transcriptional regulator RstA
Synonym(s): RstA, RstA response regulator, UrpT
Sequence: Get amino acid sequence Fasta Format
Regulator Family: OmpR
Cellular location: cytosol
Molecular weight: 26.704
Isoelectric point: 5.359
Motif(s):
 
Type Positions Sequence
158 -> 233 TEISLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLF
3 -> 116 TIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHL
4 -> 112 IVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARL
136 -> 235 YKALHFGTLTIDPINRVVTLANTEISLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAP

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
  3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.4 - complex regulation --> 3.1.2.4.3 - two component regulatory systems (external signal)
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0032993 - protein-DNA complex
molecular_function GO:0003677 - DNA binding
GO:0005515 - protein binding
GO:0003700 - DNA-binding transcription factor activity
GO:0000156 - phosphorelay response regulator activity
GO:0000976 - transcription regulatory region sequence-specific DNA binding
GO:0001216 - DNA-binding transcription activator activity
biological_process GO:0006355 - regulation of transcription, DNA-templated
GO:0045893 - positive regulation of transcription, DNA-templated
GO:0000160 - phosphorelay signal transduction system
External database links:  
DIP:
 DIP-10807N
ECOCYC:
 RSTA-MONOMER
ECOLIWIKI:
 b1608
INTERPRO:
 IPR039420
INTERPRO:
 IPR016032
INTERPRO:
 IPR011006
INTERPRO:
 IPR001789
INTERPRO:
 IPR036388
INTERPRO:
 IPR001867
MINT:
 MINT-1274913
MODBASE:
 P52108
PANTHER:
 PTHR26402
PFAM:
 PF00486
PFAM:
 PF00072
PRIDE:
 P52108
PRODB:
 PRO_000023889
PROSITE:
 PS50110
PROSITE:
 PS51755
REFSEQ:
 NP_416125
SMART:
 SM00862
SMART:
 SM00448
SMR:
 P52108
UNIPROT:
 P52108


Operon      
Name: rstAB         
Operon arrangement:
Transcription unit        Promoter
rstAB


Transcriptional Regulation      
Display Regulation             
Activated by: PhoP


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ydgCp3 1682062 reverse Similarity to the consensus
Read more > 		[ICWHO] [1]
  promoter TSS_1894 1682136 forward nd [RS-EPT-CBR] [2]
  promoter tusp2 1684173 forward Similarity to the consensus
Read more > 		[ICWHO] [1]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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