RegulonDB

Gene
Name:	bamB
Synonym(s):	ECK2508, G7320, b2512, yfgL
Genome position(nucleotides):	2637474 <-- 2638652 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.27
External database links:

ASAP:	ABE-0008272
ECHOBASE:	EB3960
OU-MICROARRAY:	b2512
PortEco:	bamB
STRING:	511145.b2512
COLOMBOS:	bamB

Product

Name:

outer membrane protein assembly factor BamB

Synonym(s):

BamB, YfgL

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

outer membrane

Molecular weight:

41.887

Isoelectric point:

4.495

Motif(s):

Type	Positions	Sequence
	78 -> 319	AGLVKALNADDGKEIWSVSLAEKDGWFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDGLVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPTTAFGAAVVGGDNGRVSAVLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQIMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQ
	20 -> 392	CSLFNSEEDVVKMSPLPTVENQFTPTTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDGWFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDGLVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPTTAFGAAVVGGDNGRVSAVLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQIMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYNGNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
	1 -> 19	MQLRKLLLPGLLSVTLLSG

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0009279 - cell outer membrane GO:0016020 - membrane GO:1990063 - Bam protein complex
	molecular_function	GO:0005515 - protein binding GO:0042802 - identical protein binding
	biological_process	GO:0043165 - Gram-negative-bacterium-type cell outer membrane assembly GO:0051205 - protein insertion into membrane

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[APPHINH] Assay of protein purified to homogeneity from its native host
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Hagan CL., et al., 2010
[2] Han L., et al., 2016
[3] Hart EM., et al., 2019
[4] Wu T., et al., 2005

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Operon

Name:

bamB-der

Operon arrangement:

Transcription unit	Promoter
bamB-der	bamBp

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2792	2636300	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2793	2636452	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2794	2637789	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2795 (cluster)	2638676	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2638675 to 2638676, where the highest frequency is at position 2636697. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[5]
promoter	TSS_2796 (cluster)	2638683	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2638682 to 2638683, where the highest frequency is at position 2636704. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[5]
promoter	TSS_2797	2639033	reverse	nd	[RS-EPT-CBR]	[5]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Hagan CL., Kim S., Kahne D., 2010, Reconstitution of outer membrane protein assembly from purified components., Science 328(5980):890-2
[2] Han L., Zheng J., Wang Y., Yang X., Liu Y., Sun C., Cao B., Zhou H., Ni D., Lou J., Zhao Y., Huang Y., 2016, Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins., Nat Struct Mol Biol 23(3):192-6
[3] Hart EM., Gupta M., Wuhr M., Silhavy TJ., 2019, The Synthetic Phenotype of Δ bamB Δ bamE Double Mutants Results from a Lethal Jamming of the Bam Complex by the Lipoprotein RcsF., MBio 10(3)
[4] Wu T., Malinverni J., Ruiz N., Kim S., Silhavy TJ., Kahne D., 2005, Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli., Cell 121(2):235-45
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.