RegulonDB

RegulonDB RegulonDB 10.8: Gene Form

yfgM gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	yfgM
Synonym(s):	ECK2509, G7321, b2513
Genome position(nucleotides):	2638663 <-- 2639283 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.76
External database links:

ASAP:	ABE-0008274
ECHOBASE:	EB3961
OU-MICROARRAY:	b2513
PortEco:	yfgM
STRING:	511145.b2513
COLOMBOS:	yfgM

Product

Name:

ancillary SecYEG translocon subunit

Synonym(s):

YfgM

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

22.176

Isoelectric point:

4.787

Motif(s):

Type	Positions	Sequence
	20 -> 44	ENGKALAVGVILGVGALIGWRYWNS
	2 -> 15	EIYENENDQVEAVK
	15 -> 205	KRFFAENGKALAVGVILGVGALIGWRYWNSHQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKNELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIVADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLS
	44 -> 206	SHQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKNELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIVADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI
	24 -> 42	ALAVGVILGVGALIGWRYW

Classification:

Multifun Terms (GenProtEC)
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0032991 - protein-containing complex GO:0016021 - integral component of membrane GO:0071575 - integral component of external side of plasma membrane
	molecular_function	GO:0005515 - protein binding GO:0044877 - protein-containing complex binding

Note(s):

Note(s): ...[more].

Evidence:

[APPHINH] Assay of protein purified to homogeneity from its native host
[IGI] Inferred from genetic interaction

Reference(s):

[1] Gotzke H., et al., 2015
[2] Gotzke H., et al., 2014
[3] Jauss B., et al., 2019
[4] Maddalo G., et al., 2011

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

yfgM

Operon arrangement:

Transcription unit	Promoter
yfGM	yfgMp3
yfgM	yfgMp2
yfgM	yfgMp1

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2794	2637789	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2795 (cluster)	2638676	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2638675 to 2638676, where the highest frequency is at position 2636697. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[5]
promoter	TSS_2796 (cluster)	2638683	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2638682 to 2638683, where the highest frequency is at position 2636704. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[5]
promoter	TSS_2797	2639033	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2798	2639305	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2799 (cluster)	2639374	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2639373 to 2639374, where the highest frequency is at position 2637395. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[5]
promoter	TSS_2800	2639941	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2801	2639974	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2802	2640203	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2803	2640283	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2804 (cluster)	2640538	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2640537 to 2640538, where the highest frequency is at position 2638559. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[5]
promoter	TSS_2805	2640541	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2806	2640554	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2807	2640563	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2808	2640565	reverse	nd	[RS-EPT-CBR]	[5]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Gotzke H., Muheim C., Altelaar AF., Heck AJ., Maddalo G., Daley DO., 2015, Identification of Putative Substrates for the Periplasmic Chaperone YfgM in Escherichia coli Using Quantitative Proteomics., Mol Cell Proteomics 14(1):216-26
[2] Gotzke H., Palombo I., Muheim C., Perrody E., Genevaux P., Kudva R., Muller M., Daley DO., 2014, YfgM is an ancillary subunit of the SecYEG translocon in Escherichia coli., J Biol Chem 289(27):19089-97
[3] Jauss B., Petriman NA., Drepper F., Franz L., Sachelaru I., Welte T., Steinberg R., Warscheid B., Koch HG., 2019, Noncompetitive binding of PpiD and YidC to the SecYEG translocon expands the global view on the SecYEG interactome in Escherichia coli., J Biol Chem 294(50):19167-19183
[4] Maddalo G., Stenberg-Bruzell F., Gotzke H., Toddo S., Bjorkholm P., Eriksson H., Chovanec P., Genevaux P., Lehtio J., Ilag LL., Daley DO., 2011, Systematic analysis of native membrane protein complexes in Escherichia coli., J Proteome Res 10(4):1848-59
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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