RegulonDB RegulonDB 11.2: Gene Form
   

iscS gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

iscS iscR iscU FnrS Cis-reg TSS_2830 TSS_2830 TSS_2829 (cluster) TSS_2829 (cluster) TSS_2828 TSS_2828 TSS_2827 TSS_2827 iscAp6 iscAp6 iscAp7 iscAp7

Gene      
Name: iscS    Texpresso search in the literature
Synonym(s): ECK2527, G7325, b2530, nuvC, yfhO, yzzO
Genome position(nucleotides): 2660317 <-- 2661531
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.33
External database links:  
ASAP:
 ABE-0008324
ECHOBASE:
 EB2542
ECOLIHUB:
 iscS
OU-MICROARRAY:
 b2530
STRING:
 511145.b2530
COLOMBOS: iscS


Product      
Name: cysteine desulfurase IscS
Synonym(s): IscS, NuvC, YfhO, YzzO
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 45.089
Isoelectric point: 6.326
Motif(s):
 
Type Positions Sequence Comment
5 -> 368 IYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFGWQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKTEHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVVQDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNRLWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDY
31 -> 31 G UniProt: In Ref. 4; AA sequence..
34 -> 34 G UniProt: In Ref. 4; AA sequence..
37 -> 38 AS UniProt: In Ref. 4; AA sequence..
75 -> 76 AT UniProt: Pyridoxal phosphate binding; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:1990228 - sulfurtransferase complex
GO:1990329 - IscS-TusA complex
GO:1990221 - L-cysteine desulfurase complex
GO:1990330 - IscS-IscU complex
GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0009000 - selenocysteine lyase activity
GO:0031071 - cysteine desulfurase activity
GO:0030170 - pyridoxal phosphate binding
GO:0051536 - iron-sulfur cluster binding
GO:0051537 - 2 iron, 2 sulfur cluster binding
GO:0097163 - sulfur carrier activity
biological_process GO:0016261 - selenocysteine catabolic process
GO:0009589 - detection of UV
GO:0009228 - thiamine biosynthetic process
GO:0016226 - iron-sulfur cluster assembly
GO:0018131 - oxazole or thiazole biosynthetic process
GO:0008033 - tRNA processing
GO:0072348 - sulfur compound transport
GO:0002143 - tRNA wobble position uridine thiolation
GO:0002937 - tRNA 4-thiouridine biosynthesis
GO:0044571 - [2Fe-2S] cluster assembly
GO:0019448 - L-cysteine catabolic process
Reference(s): [1] Frazzon J., et al., 2001
[2] Mihara H., et al., 2002
External database links:  
ALPHAFOLD:
 P0A6B7
DIP:
 DIP-29109N
ECOCYC:
 G7325-MONOMER
ECOLIWIKI:
 b2530
INTERPRO:
 IPR010240
INTERPRO:
 IPR015421
INTERPRO:
 IPR000192
INTERPRO:
 IPR015422
INTERPRO:
 IPR020578
INTERPRO:
 IPR016454
INTERPRO:
 IPR015424
MINT:
 P0A6B7
MODBASE:
 P0A6B7
PANTHER:
 PTHR11601:SF34
PDB:
 1P3W
PFAM:
 PF00266
PRIDE:
 P0A6B7
PROSITE:
 PS00595
REFSEQ:
 YP_026169
SMR:
 P0A6B7
UNIPROT:
 P0A6B7


Operon      
Name: iscRSUA         
Operon arrangement:
Transcription unit        Promoter
iscRSUA


Transcriptional Regulation      
Display Regulation             
Repressed by: IscR


RNA cis-regulatory element    
   
Cis-reg iscR stability element
   
   


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter iscAp7 2659953 reverse nd [COMP-AINF] [3]
  promoter iscAp6 2659962 reverse nd [COMP-AINF] [3]
  promoter TSS_2827 2661124 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2828 2661560 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2829 (cluster) 2661634 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2830 2661996 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Frazzon J., Dean DR., 2001, Feedback regulation of iron-sulfur cluster biosynthesis., Proc Natl Acad Sci U S A 98(26):14751-3

 [2] Mihara H., Esaki N., 2002, Bacterial cysteine desulfurases: their function and mechanisms., Appl Microbiol Biotechnol 60(1-2):12-23

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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