RegulonDB

Gene
Name:	nrdI
Synonym(s):	ECK2668, G7402, b2674, ygaO
Genome position(nucleotides):	2800965 --> 2801375
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.96
External database links:

ASAP:	ABE-0008804
ECHOBASE:	EB3072
ECOLIHUB:	nrdI
OU-MICROARRAY:	b2674
STRING:	511145.b2674
COLOMBOS:	nrdI

Product

Name:

dimanganese-tyrosyl radical cofactor maintenance flavodoxin NrdI

Synonym(s):

NrdI, YgaO, flavodoxin NrdI, flavodoxin involved in dimanganese-tyrosyl radical cofactor maintenance for ribonucleotide reductase

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

15.34

Isoelectric point:

9.531

Motif(s):

Type	Positions	Sequence	Comment
	5 -> 122	VYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYILIVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCGVPWLYRFELMGTQSDIENVR

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		2 - information transfer --> 2.3 - protein related --> 2.3.3 - posttranslational modification
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0010181 - FMN binding
	biological_process	GO:0006464 - cellular protein modification process

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IDA-PURIFIED-PROTEIN] Assay of protein purified to homogeneity

Reference(s):

[1] Cotruvo JA., et al., 2008
[2] Cotruvo JA., et al., 2011

External database links:

ALPHAFOLD:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

nrdHIEF

Operon arrangement:

Transcription unit	Promoter
nrdHIEF	nrdHp

Transcriptional Regulation
	Display Regulation
Activated by:	IscR
Repressed by:	Fur, NrdR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Cotruvo JA., Stubbe J., 2008, NrdI, a flavodoxin involved in maintenance of the diferric-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase., Proc Natl Acad Sci U S A 105(38):14383-8
[2] Cotruvo JA., Stubbe J., 2011, Escherichia coli Class Ib Ribonucleotide Reductase Contains a Dimanganese(III)-Tyrosyl Radical Cofactor in Vivo., Biochemistry 50(10):1672-81