RegulonDB

Gene
Name:	btsT
Synonym(s):	ECK4344, G7942, b4354, yjiY
Genome position(nucleotides):	4589129 <-- 4591279 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	56.07
External database links:

ASAP:	ABE-0014278
ECHOBASE:	EB2472
ECOLIHUB:	yjiY
OU-MICROARRAY:	b4354
STRING:	511145.b4354
COLOMBOS:	btsT

Product

Name:

pyruvate:H⁺ symporter

Synonym(s):

BtsT, YjiY

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

77.324

Isoelectric point:

8.931

Motif(s):

Type	Positions	Sequence
	283 -> 303	YLATFLKIGVIVGLALGIVVL
	34 -> 405	ISALWIVVASVSVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAGPLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPVPGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPGRVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWLILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFITIACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLYFAMNTPPAGLGIT
	487 -> 614	GFWYHFGILFEALFILTALDAGTRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLWPLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWA
	375 -> 395	FVAIMALVAASIIEPGLYFAM
	464 -> 484	GGAPTLAVGIAHVFHKVLPMA

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0005477 - pyruvate secondary active transmembrane transporter activity GO:0005515 - protein binding GO:0015293 - symporter activity GO:0042802 - identical protein binding
	biological_process	GO:0006849 - plasma membrane pyruvate transport GO:0006974 - cellular response to DNA damage stimulus GO:0009267 - cellular response to starvation GO:0031669 - cellular response to nutrient levels

Note(s):

Note(s): ...[more].

Reference(s):

[1] Gasperotti A., et al., 2020
[2] Hwang S., et al., 2018
[3] Kornberg HL., et al., 1967
[4] Kreth J., et al., 2013
[5] Lang VJ., et al., 1987
[6] Matin A., et al., 1973
[7] Vilhena C., et al., 2019
[8] Zhao T., et al., 2021

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PFAM:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

btsT

Operon arrangement:

Transcription unit	Promoter
btsT	yjiYp

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, BtsR

RNA cis-regulatory element

Attenuation:	Translational

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yjiAp6	4588995	reverse	nd	[ICWHO]	[9]
promoter	yjiAp7	4589048	reverse	nd	[ICWHO]	[9]
promoter	yjiYp3	4591347	reverse	nd	[ICWHO]	[9]

Evidence
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Gasperotti A., Going S., Fajardo-Ruiz E., Forne I., Jung K., 2020, Function and Regulation of the Pyruvate Transporter CstA in Escherichia coli., Int J Mol Sci 21(23)
[2] Hwang S., Choe D., Yoo M., Kim SC., Cho S., Cho BK., 2018, Peptide Transporter CstA Imports Pyruvate in Escherichia coli K-12., J Bacteriol 200(7)
[3] Kornberg HL., Smith J., 1967, Genetic control of the uptake of pyruvate by Escherichia coli., Biochim Biophys Acta 148(2):591-2
[4] Kreth J., Lengeler JW., Jahreis K., 2013, Characterization of pyruvate uptake in Escherichia coli K-12., PLoS One 8(6):e67125
[5] Lang VJ., Leystra-Lantz C., Cook RA., 1987, Characterization of the specific pyruvate transport system in Escherichia coli K-12., J Bacteriol 169(1):380-5
[6] Matin A., Konings WN., 1973, Transport of lactate and succinate by membrane vesicles of Escherichia coli, Bacillus subtilis and a pseudomonas species., Eur J Biochem 34(1):58-67
[7] Vilhena C., Kaganovitch E., Grunberger A., Motz M., Forne I., Kohlheyer D., Jung K., 2019, Importance of Pyruvate Sensing and Transport for the Resuscitation of Viable but Nonculturable Escherichia coli K-12., J Bacteriol 201(3)
[8] Zhao T., Xu L., Zhao L., Zhang H., Li Y., Zhang Y., 2021, BtsT/ BtsS is involved in glyoxylate transport in E. coli and its mutations facilitated glyoxylate utilization., Biochem Biophys Res Commun 551:71-77
[9] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78