RegulonDB RegulonDB 11.0: Gene Form
   

ampC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ampC blc frdD gdx anti-anti-terminator anti-terminator terminator anti-anti-terminator anti-terminator terminator TSS_4970 (cluster) TSS_4970 (cluster) TSS_4969 TSS_4969 TSS_4968 TSS_4968 ampCp ampCp blcp blcp

Gene      
Name: ampC    Texpresso search in the literature
Synonym(s): ECK4146, EG10040, ampA, b4150
Genome position(nucleotides): 4377811 <-- 4378944
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
50.18
External database links:  
ASAP:
ABE-0013592
CGSC:
1033
ECHOBASE:
EB0038
ECOLIHUB:
ampC
OU-MICROARRAY:
b4150
STRING:
511145.b4150
COLOMBOS: ampC


Product      
Name: β-lactamase
Synonym(s): AmpA, AmpC
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 41.556
Isoelectric point: 9.077
Motif(s):
 
Type Positions Sequence Comment
1 -> 19 MFKTTLCALLITASCSTFA
20 -> 377 APQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNALQ UniProt: Beta-lactamase.
30 -> 376 RTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNYPNPARVDAAWQILNAL
80 -> 80 S UniProt: Loss of activity..
83 -> 83 K UniProt: Lowers activity more than 1000-fold and increases protein stability..

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.7 - murein (peptidoglycan)
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.2 - murein
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
molecular_function GO:0016787 - hydrolase activity
GO:0008800 - beta-lactamase activity
biological_process GO:0046677 - response to antibiotic
GO:0017001 - antibiotic catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Levin-Reisman I., et al., 2017
External database links:  
ALPHAFOLD:
P00811
ECOCYC:
EG10040-MONOMER
ECOLIWIKI:
b4150
INTERPRO:
IPR012338
INTERPRO:
IPR001466
INTERPRO:
IPR001586
MODBASE:
P00811
PDB:
1C3B
PDB:
1FCM
PDB:
1FCN
PDB:
1FCO
PDB:
1FSW
PDB:
1FSY
PDB:
1GA9
PDB:
1I5Q
PDB:
1IEL
PDB:
1IEM
PDB:
1KDS
PDB:
1KDW
PDB:
1KE0
PDB:
1KE3
PDB:
1KE4
PDB:
1KVL
PDB:
1KVM
PDB:
1L0D
PDB:
1L0E
PDB:
1L0F
PDB:
1L0G
PDB:
1L2S
PDB:
1LL5
PDB:
1LL9
PDB:
1LLB
PDB:
1MXO
PDB:
1MY8
PDB:
1O07
PDB:
1PI4
PDB:
1PI5
PDB:
1XGI
PDB:
1XGJ
PDB:
2BLS
PDB:
2FFY
PDB:
2HDQ
PDB:
2HDR
PDB:
2HDS
PDB:
2HDU
PDB:
2I72
PDB:
2P9V
PDB:
2PU2
PDB:
2PU4
PDB:
2R9W
PDB:
2R9X
PDB:
2RCX
PDB:
3BLS
PDB:
3BM6
PDB:
3FKV
PDB:
3FKW
PDB:
3GQZ
PDB:
3GR2
PDB:
3GRJ
PDB:
3GSG
PDB:
3GTC
PDB:
3GV9
PDB:
3GVB
PDB:
3IWI
PDB:
3IWO
PDB:
3IWQ
PDB:
3IXB
PDB:
3IXD
PDB:
3IXG
PDB:
3IXH
PDB:
3O86
PDB:
3O87
PDB:
3O88
PDB:
4E3I
PDB:
4E3J
PDB:
4E3K
PDB:
4E3L
PDB:
4E3M
PDB:
4E3N
PDB:
4E3O
PDB:
4JXG
PDB:
4JXS
PDB:
4JXV
PDB:
4JXW
PDB:
4KEN
PDB:
4KG2
PDB:
4KG5
PDB:
4KG6
PDB:
4KZ3
PDB:
4KZ4
PDB:
4KZ5
PDB:
4KZ6
PDB:
4KZ7
PDB:
4KZ8
PDB:
4KZ9
PDB:
4KZA
PDB:
4KZB
PDB:
4LV0
PDB:
4LV1
PDB:
4LV2
PDB:
4LV3
PDB:
4OKP
PDB:
4OLD
PDB:
4OLG
PDB:
5JOC
PDB:
6DPT
PDB:
6DPX
PDB:
6DPY
PDB:
6DPZ
PDB:
6T35
PDB:
6T3D
PDB:
6T5Y
PDB:
6T7L
PDB:
6TBW
PDB:
6TPM
PDB:
6WHF
PDB:
6X9Y
PDB:
6XFS
PDB:
6XG1
PDB:
6YEN
PDB:
6YEO
PDB:
6YPD
PFAM:
PF00144
PRIDE:
P00811
PRODB:
PRO_000022095
PROSITE:
PS00336
REFSEQ:
NP_418574
SMR:
P00811
UNIPROT:
P00811


Operon      
Name: ampC         
Operon arrangement:
Transcription unit        Promoter
ampC


Transcriptional Regulation      
Display Regulation             
Activated by: BolA
Repressed by: BolA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4968 4379152 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_4969 4379156 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_4970 (cluster) 4379163 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Levin-Reisman I., Ronin I., Gefen O., Braniss I., Shoresh N., Balaban NQ., 2017, Antibiotic tolerance facilitates the evolution of resistance., Science 355(6327):826-830

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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